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Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases
BACKGROUND: In Brazil, few studies have investigated the prevalence of infection with the precore (PC) and basal core promoter (BCP) mutants of the hepatitis B virus (HBV). OBJECTIVES: This study aimed to analyse the frequency of PC and BCP mutations among patients infected with HBV and to evaluate...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572448/ https://www.ncbi.nlm.nih.gov/pubmed/28902288 http://dx.doi.org/10.1590/0074-02760160540 |
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author | Chachá, Silvana Gama Florencio Gomes-Gouvêa, Michele Soares Malta, Fernanda de Mello Ferreira, Sandro da Costa Villanova, Márcia Guimarães Souza, Fernanda Fernandes Teixeira, Andreza Correa Passos, Afonso Dinis da Costa Pinho, João Renato Rebello Martinelli, Ana de Lourdes Candolo |
author_facet | Chachá, Silvana Gama Florencio Gomes-Gouvêa, Michele Soares Malta, Fernanda de Mello Ferreira, Sandro da Costa Villanova, Márcia Guimarães Souza, Fernanda Fernandes Teixeira, Andreza Correa Passos, Afonso Dinis da Costa Pinho, João Renato Rebello Martinelli, Ana de Lourdes Candolo |
author_sort | Chachá, Silvana Gama Florencio |
collection | PubMed |
description | BACKGROUND: In Brazil, few studies have investigated the prevalence of infection with the precore (PC) and basal core promoter (BCP) mutants of the hepatitis B virus (HBV). OBJECTIVES: This study aimed to analyse the frequency of PC and BCP mutations among patients infected with HBV and to evaluate the association between the variants and advanced hepatic disease. METHODS: A total of 161 patients infected with HBV were studied. To identify PC and BCP mutations, a 501-bp fragment of HBV DNA was amplified and sequenced. FINDINGS: PC and BCP regions from HBV strains were successfully amplified and sequenced in 129 and 118 cases, respectively. PC and BCP mutations were detected in 61.0% and 80.6% of the cases, respectively. The A1762T/G1764A variant was identified in 36.7% of the patients with grade 1 and 2 liver fibrosis (29/79) and in 81.8% of the patients with grade 3 and 4 liver fibrosis (9/11) (p < 0.01); in 76.9% of the patients with cirrhosis (10/13) and in 38.1% of the patients without cirrhosis (40/105) (p = 0.01); and in 77.8% of the patients with hepatocellular carcinoma (HCC) (7/9) and in 39.4% of the patients without HCC (43/109) (p = 0.03). MAIN CONCLUSIONS: A high prevalence of HBV PC and BCP mutants was found. The A1762T/G1764A variant was independently associated with advanced forms of liver fibrosis, hepatic cirrhosis, and HCC. |
format | Online Article Text |
id | pubmed-5572448 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-55724482017-09-01 Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases Chachá, Silvana Gama Florencio Gomes-Gouvêa, Michele Soares Malta, Fernanda de Mello Ferreira, Sandro da Costa Villanova, Márcia Guimarães Souza, Fernanda Fernandes Teixeira, Andreza Correa Passos, Afonso Dinis da Costa Pinho, João Renato Rebello Martinelli, Ana de Lourdes Candolo Mem Inst Oswaldo Cruz Articles BACKGROUND: In Brazil, few studies have investigated the prevalence of infection with the precore (PC) and basal core promoter (BCP) mutants of the hepatitis B virus (HBV). OBJECTIVES: This study aimed to analyse the frequency of PC and BCP mutations among patients infected with HBV and to evaluate the association between the variants and advanced hepatic disease. METHODS: A total of 161 patients infected with HBV were studied. To identify PC and BCP mutations, a 501-bp fragment of HBV DNA was amplified and sequenced. FINDINGS: PC and BCP regions from HBV strains were successfully amplified and sequenced in 129 and 118 cases, respectively. PC and BCP mutations were detected in 61.0% and 80.6% of the cases, respectively. The A1762T/G1764A variant was identified in 36.7% of the patients with grade 1 and 2 liver fibrosis (29/79) and in 81.8% of the patients with grade 3 and 4 liver fibrosis (9/11) (p < 0.01); in 76.9% of the patients with cirrhosis (10/13) and in 38.1% of the patients without cirrhosis (40/105) (p = 0.01); and in 77.8% of the patients with hepatocellular carcinoma (HCC) (7/9) and in 39.4% of the patients without HCC (43/109) (p = 0.03). MAIN CONCLUSIONS: A high prevalence of HBV PC and BCP mutants was found. The A1762T/G1764A variant was independently associated with advanced forms of liver fibrosis, hepatic cirrhosis, and HCC. Instituto Oswaldo Cruz, Ministério da Saúde 2017-09 /pmc/articles/PMC5572448/ /pubmed/28902288 http://dx.doi.org/10.1590/0074-02760160540 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Chachá, Silvana Gama Florencio Gomes-Gouvêa, Michele Soares Malta, Fernanda de Mello Ferreira, Sandro da Costa Villanova, Márcia Guimarães Souza, Fernanda Fernandes Teixeira, Andreza Correa Passos, Afonso Dinis da Costa Pinho, João Renato Rebello Martinelli, Ana de Lourdes Candolo Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases |
title | Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases |
title_full | Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases |
title_fullStr | Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases |
title_full_unstemmed | Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases |
title_short | Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases |
title_sort | basal core promoter and precore mutations among hepatitis b virus circulating in brazil and its association with severe forms of hepatic diseases |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572448/ https://www.ncbi.nlm.nih.gov/pubmed/28902288 http://dx.doi.org/10.1590/0074-02760160540 |
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