Cargando…

Evaluation of 5 Commercially Available Zika Virus Immunoassays

Because of the global spread of Zika virus, accurate and high-throughput diagnostic immunoassays are needed. We compared the sensitivity and specificity of 5 commercially available Zika virus serologic assays to the recommended protocol of Zika virus IgM-capture ELISA and plaque-reduction neutraliza...

Descripción completa

Detalles Bibliográficos
Autores principales: Safronetz, David, Sloan, Angela, Stein, Derek R., Mendoza, Emelissa, Barairo, Nicole, Ranadheera, Charlene, Scharikow, Leanne, Holloway, Kimberly, Robinson, Alyssia, Traykova-Andonova, Maya, Makowski, Kai, Dimitrova, Kristina, Giles, Elizabeth, Hiebert, Joanne, Mogk, Rhonda, Beddome, Sharla, Drebot, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572859/
https://www.ncbi.nlm.nih.gov/pubmed/28665268
http://dx.doi.org/10.3201/eid2309.162043
_version_ 1783259575679451136
author Safronetz, David
Sloan, Angela
Stein, Derek R.
Mendoza, Emelissa
Barairo, Nicole
Ranadheera, Charlene
Scharikow, Leanne
Holloway, Kimberly
Robinson, Alyssia
Traykova-Andonova, Maya
Makowski, Kai
Dimitrova, Kristina
Giles, Elizabeth
Hiebert, Joanne
Mogk, Rhonda
Beddome, Sharla
Drebot, Michael
author_facet Safronetz, David
Sloan, Angela
Stein, Derek R.
Mendoza, Emelissa
Barairo, Nicole
Ranadheera, Charlene
Scharikow, Leanne
Holloway, Kimberly
Robinson, Alyssia
Traykova-Andonova, Maya
Makowski, Kai
Dimitrova, Kristina
Giles, Elizabeth
Hiebert, Joanne
Mogk, Rhonda
Beddome, Sharla
Drebot, Michael
author_sort Safronetz, David
collection PubMed
description Because of the global spread of Zika virus, accurate and high-throughput diagnostic immunoassays are needed. We compared the sensitivity and specificity of 5 commercially available Zika virus serologic assays to the recommended protocol of Zika virus IgM-capture ELISA and plaque-reduction neutralization tests. Most commercial immunoassays showed low sensitivity, which can be increased.
format Online
Article
Text
id pubmed-5572859
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Centers for Disease Control and Prevention
record_format MEDLINE/PubMed
spelling pubmed-55728592017-09-02 Evaluation of 5 Commercially Available Zika Virus Immunoassays Safronetz, David Sloan, Angela Stein, Derek R. Mendoza, Emelissa Barairo, Nicole Ranadheera, Charlene Scharikow, Leanne Holloway, Kimberly Robinson, Alyssia Traykova-Andonova, Maya Makowski, Kai Dimitrova, Kristina Giles, Elizabeth Hiebert, Joanne Mogk, Rhonda Beddome, Sharla Drebot, Michael Emerg Infect Dis Dispatch Because of the global spread of Zika virus, accurate and high-throughput diagnostic immunoassays are needed. We compared the sensitivity and specificity of 5 commercially available Zika virus serologic assays to the recommended protocol of Zika virus IgM-capture ELISA and plaque-reduction neutralization tests. Most commercial immunoassays showed low sensitivity, which can be increased. Centers for Disease Control and Prevention 2017-09 /pmc/articles/PMC5572859/ /pubmed/28665268 http://dx.doi.org/10.3201/eid2309.162043 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Safronetz, David
Sloan, Angela
Stein, Derek R.
Mendoza, Emelissa
Barairo, Nicole
Ranadheera, Charlene
Scharikow, Leanne
Holloway, Kimberly
Robinson, Alyssia
Traykova-Andonova, Maya
Makowski, Kai
Dimitrova, Kristina
Giles, Elizabeth
Hiebert, Joanne
Mogk, Rhonda
Beddome, Sharla
Drebot, Michael
Evaluation of 5 Commercially Available Zika Virus Immunoassays
title Evaluation of 5 Commercially Available Zika Virus Immunoassays
title_full Evaluation of 5 Commercially Available Zika Virus Immunoassays
title_fullStr Evaluation of 5 Commercially Available Zika Virus Immunoassays
title_full_unstemmed Evaluation of 5 Commercially Available Zika Virus Immunoassays
title_short Evaluation of 5 Commercially Available Zika Virus Immunoassays
title_sort evaluation of 5 commercially available zika virus immunoassays
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572859/
https://www.ncbi.nlm.nih.gov/pubmed/28665268
http://dx.doi.org/10.3201/eid2309.162043
work_keys_str_mv AT safronetzdavid evaluationof5commerciallyavailablezikavirusimmunoassays
AT sloanangela evaluationof5commerciallyavailablezikavirusimmunoassays
AT steinderekr evaluationof5commerciallyavailablezikavirusimmunoassays
AT mendozaemelissa evaluationof5commerciallyavailablezikavirusimmunoassays
AT baraironicole evaluationof5commerciallyavailablezikavirusimmunoassays
AT ranadheeracharlene evaluationof5commerciallyavailablezikavirusimmunoassays
AT scharikowleanne evaluationof5commerciallyavailablezikavirusimmunoassays
AT hollowaykimberly evaluationof5commerciallyavailablezikavirusimmunoassays
AT robinsonalyssia evaluationof5commerciallyavailablezikavirusimmunoassays
AT traykovaandonovamaya evaluationof5commerciallyavailablezikavirusimmunoassays
AT makowskikai evaluationof5commerciallyavailablezikavirusimmunoassays
AT dimitrovakristina evaluationof5commerciallyavailablezikavirusimmunoassays
AT gileselizabeth evaluationof5commerciallyavailablezikavirusimmunoassays
AT hiebertjoanne evaluationof5commerciallyavailablezikavirusimmunoassays
AT mogkrhonda evaluationof5commerciallyavailablezikavirusimmunoassays
AT beddomesharla evaluationof5commerciallyavailablezikavirusimmunoassays
AT drebotmichael evaluationof5commerciallyavailablezikavirusimmunoassays