Rational identification of aggregation hotspots based on secondary structure and amino acid hydrophobicity

Insolubility of proteins expressed in the Escherichia coli expression system hinders the progress of both basic and applied research. Insoluble proteins contain residues that decrease their solubility (aggregation hotspots). Mutating these hotspots to optimal amino acids is expected to improve prote...

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Detalles Bibliográficos
Autores principales: Matsui, Daisuke, Nakano, Shogo, Dadashipour, Mohammad, Asano, Yasuhisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573320/
https://www.ncbi.nlm.nih.gov/pubmed/28842596
http://dx.doi.org/10.1038/s41598-017-09749-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573320/
https://www.ncbi.nlm.nih.gov/pubmed/28842596
http://dx.doi.org/10.1038/s41598-017-09749-2

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