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Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule

The CRISPR/Cas9 system has been applied in diverse eukaryotic organisms for targeted mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous delivery of a DNA template for homology-directed repair (HDR). Here, we used CRISPR/Cas9 to generate targeted double-strand br...

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Detalles Bibliográficos
Autores principales:	Butt, Haroon, Eid, Ayman, Ali, Zahir, Atia, Mohamed A. M., Mokhtar, Morad M., Hassan, Norhan, Lee, Ciaran M., Bao, Gang, Mahfouz, Magdy M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2017
Materias:	Plant Science
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573723/ https://www.ncbi.nlm.nih.gov/pubmed/28883826 http://dx.doi.org/10.3389/fpls.2017.01441

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