Multiplex PCR assay for the simultaneous detection and differentiation of clonal lineages of Erysipelothrix rhusiopathiae serovar 1a strains currently circulating in Japan

The species Erysipelothrix rhusiopathiae displays genetic heterogeneity; however, E. rhusiopathiae serovar 1a strains currently circulating in Japan exhibit remarkably low levels of genetic diversity and group into clonal sublineages of Lineage IVb (IVb-1 and IVb-2). In the present study, based on w...

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Autores principales: SHIRAIWA, Kazumasa, OGAWA, Yohsuke, NISHIKAWA, Sayaka, EGUCHI, Masahiro, SHIMOJI, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2017
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573815/
https://www.ncbi.nlm.nih.gov/pubmed/28637945
http://dx.doi.org/10.1292/jvms.17-0255
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author SHIRAIWA, Kazumasa
OGAWA, Yohsuke
NISHIKAWA, Sayaka
EGUCHI, Masahiro
SHIMOJI, Yoshihiro
author_facet SHIRAIWA, Kazumasa
OGAWA, Yohsuke
NISHIKAWA, Sayaka
EGUCHI, Masahiro
SHIMOJI, Yoshihiro
author_sort SHIRAIWA, Kazumasa
collection PubMed
description The species Erysipelothrix rhusiopathiae displays genetic heterogeneity; however, E. rhusiopathiae serovar 1a strains currently circulating in Japan exhibit remarkably low levels of genetic diversity and group into clonal sublineages of Lineage IVb (IVb-1 and IVb-2). In the present study, based on whole genome sequencing data, we designed primers for a multiplex PCR assay to simultaneously detect and differentiate the sublineages of E. rhusiopathiae strains. Among the one hundred and twenty-seven isolates of various serovar strains, including isolates from a wide range of hosts and geographic origins, the PCR assay could successfully detect and differentiate the serovar 1a strains belonging to the sublineages.
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spelling pubmed-55738152017-08-29 Multiplex PCR assay for the simultaneous detection and differentiation of clonal lineages of Erysipelothrix rhusiopathiae serovar 1a strains currently circulating in Japan SHIRAIWA, Kazumasa OGAWA, Yohsuke NISHIKAWA, Sayaka EGUCHI, Masahiro SHIMOJI, Yoshihiro J Vet Med Sci Bacteriology The species Erysipelothrix rhusiopathiae displays genetic heterogeneity; however, E. rhusiopathiae serovar 1a strains currently circulating in Japan exhibit remarkably low levels of genetic diversity and group into clonal sublineages of Lineage IVb (IVb-1 and IVb-2). In the present study, based on whole genome sequencing data, we designed primers for a multiplex PCR assay to simultaneously detect and differentiate the sublineages of E. rhusiopathiae strains. Among the one hundred and twenty-seven isolates of various serovar strains, including isolates from a wide range of hosts and geographic origins, the PCR assay could successfully detect and differentiate the serovar 1a strains belonging to the sublineages. The Japanese Society of Veterinary Science 2017-06-22 2017-08 /pmc/articles/PMC5573815/ /pubmed/28637945 http://dx.doi.org/10.1292/jvms.17-0255 Text en ©2017 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Bacteriology
SHIRAIWA, Kazumasa
OGAWA, Yohsuke
NISHIKAWA, Sayaka
EGUCHI, Masahiro
SHIMOJI, Yoshihiro
Multiplex PCR assay for the simultaneous detection and differentiation of clonal lineages of Erysipelothrix rhusiopathiae serovar 1a strains currently circulating in Japan
title Multiplex PCR assay for the simultaneous detection and differentiation of clonal lineages of Erysipelothrix rhusiopathiae serovar 1a strains currently circulating in Japan
title_full Multiplex PCR assay for the simultaneous detection and differentiation of clonal lineages of Erysipelothrix rhusiopathiae serovar 1a strains currently circulating in Japan
title_fullStr Multiplex PCR assay for the simultaneous detection and differentiation of clonal lineages of Erysipelothrix rhusiopathiae serovar 1a strains currently circulating in Japan
title_full_unstemmed Multiplex PCR assay for the simultaneous detection and differentiation of clonal lineages of Erysipelothrix rhusiopathiae serovar 1a strains currently circulating in Japan
title_short Multiplex PCR assay for the simultaneous detection and differentiation of clonal lineages of Erysipelothrix rhusiopathiae serovar 1a strains currently circulating in Japan
title_sort multiplex pcr assay for the simultaneous detection and differentiation of clonal lineages of erysipelothrix rhusiopathiae serovar 1a strains currently circulating in japan
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573815/
https://www.ncbi.nlm.nih.gov/pubmed/28637945
http://dx.doi.org/10.1292/jvms.17-0255
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