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Defect of rabies virus phosphoprotein in its interferon-antagonist activity negatively affects viral replication in muscle cells

Attenuated derivative rabies virus Ni-CE replicates in muscle cells less efficiently than does the parental pathogenic strain Nishigahara. To examine the mechanism underlying the less efficient replication of Ni-CE, we compared the activities of Ni-CE and Nishigahara phosphoproteins, viral interfero...

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Autores principales: YAMAOKA, Satoko, OKADA, Kazuma, ITO, Naoto, OKADERA, Kota, MITAKE, Hiromichi, NAKAGAWA, Kento, SUGIYAMA, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573828/
https://www.ncbi.nlm.nih.gov/pubmed/28674326
http://dx.doi.org/10.1292/jvms.17-0054
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author YAMAOKA, Satoko
OKADA, Kazuma
ITO, Naoto
OKADERA, Kota
MITAKE, Hiromichi
NAKAGAWA, Kento
SUGIYAMA, Makoto
author_facet YAMAOKA, Satoko
OKADA, Kazuma
ITO, Naoto
OKADERA, Kota
MITAKE, Hiromichi
NAKAGAWA, Kento
SUGIYAMA, Makoto
author_sort YAMAOKA, Satoko
collection PubMed
description Attenuated derivative rabies virus Ni-CE replicates in muscle cells less efficiently than does the parental pathogenic strain Nishigahara. To examine the mechanism underlying the less efficient replication of Ni-CE, we compared the activities of Ni-CE and Nishigahara phosphoproteins, viral interferon (IFN) antagonists, to suppress IFN-β promoter activity in muscle cells and we demonstrated a defect of Ni-CE phosphoprotein in this ability. Treatment with an IFN-β-neutralizing antibody improved the replication efficiency of Ni-CE in muscle cells, indicating that produced IFN inhibits Ni-CE replication. The results indicate the importance of IFN antagonism of rabies virus phosphoprotein for viral replication in muscle cells.
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spelling pubmed-55738282017-08-29 Defect of rabies virus phosphoprotein in its interferon-antagonist activity negatively affects viral replication in muscle cells YAMAOKA, Satoko OKADA, Kazuma ITO, Naoto OKADERA, Kota MITAKE, Hiromichi NAKAGAWA, Kento SUGIYAMA, Makoto J Vet Med Sci Public Health Attenuated derivative rabies virus Ni-CE replicates in muscle cells less efficiently than does the parental pathogenic strain Nishigahara. To examine the mechanism underlying the less efficient replication of Ni-CE, we compared the activities of Ni-CE and Nishigahara phosphoproteins, viral interferon (IFN) antagonists, to suppress IFN-β promoter activity in muscle cells and we demonstrated a defect of Ni-CE phosphoprotein in this ability. Treatment with an IFN-β-neutralizing antibody improved the replication efficiency of Ni-CE in muscle cells, indicating that produced IFN inhibits Ni-CE replication. The results indicate the importance of IFN antagonism of rabies virus phosphoprotein for viral replication in muscle cells. The Japanese Society of Veterinary Science 2017-06-30 2017-08 /pmc/articles/PMC5573828/ /pubmed/28674326 http://dx.doi.org/10.1292/jvms.17-0054 Text en ©2017 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Public Health
YAMAOKA, Satoko
OKADA, Kazuma
ITO, Naoto
OKADERA, Kota
MITAKE, Hiromichi
NAKAGAWA, Kento
SUGIYAMA, Makoto
Defect of rabies virus phosphoprotein in its interferon-antagonist activity negatively affects viral replication in muscle cells
title Defect of rabies virus phosphoprotein in its interferon-antagonist activity negatively affects viral replication in muscle cells
title_full Defect of rabies virus phosphoprotein in its interferon-antagonist activity negatively affects viral replication in muscle cells
title_fullStr Defect of rabies virus phosphoprotein in its interferon-antagonist activity negatively affects viral replication in muscle cells
title_full_unstemmed Defect of rabies virus phosphoprotein in its interferon-antagonist activity negatively affects viral replication in muscle cells
title_short Defect of rabies virus phosphoprotein in its interferon-antagonist activity negatively affects viral replication in muscle cells
title_sort defect of rabies virus phosphoprotein in its interferon-antagonist activity negatively affects viral replication in muscle cells
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573828/
https://www.ncbi.nlm.nih.gov/pubmed/28674326
http://dx.doi.org/10.1292/jvms.17-0054
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