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Single-Molecule Plasmon Sensing: Current Status and Future Prospects

[Image: see text] Single-molecule detection has long relied on fluorescent labeling with high quantum-yield fluorophores. Plasmon-enhanced detection circumvents the need for labeling by allowing direct optical detection of weakly emitting and completely nonfluorescent species. This review focuses on...

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Autores principales: Taylor, Adam B., Zijlstra, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573902/
https://www.ncbi.nlm.nih.gov/pubmed/28762723
http://dx.doi.org/10.1021/acssensors.7b00382
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author Taylor, Adam B.
Zijlstra, Peter
author_facet Taylor, Adam B.
Zijlstra, Peter
author_sort Taylor, Adam B.
collection PubMed
description [Image: see text] Single-molecule detection has long relied on fluorescent labeling with high quantum-yield fluorophores. Plasmon-enhanced detection circumvents the need for labeling by allowing direct optical detection of weakly emitting and completely nonfluorescent species. This review focuses on recent advances in single molecule detection using plasmonic metal nanostructures as a sensing platform, particularly using a single particle–single molecule approach. In the past decade two mechanisms for plasmon-enhanced single-molecule detection have been demonstrated: (1) by plasmonically enhancing the emission of weakly fluorescent biomolecules, or (2) by monitoring shifts of the plasmon resonance induced by single-molecule interactions. We begin with a motivation regarding the importance of single molecule detection, and advantages plasmonic detection offers. We describe both detection mechanisms and discuss challenges and potential solutions. We finalize by highlighting the exciting possibilities in analytical chemistry and medical diagnostics.
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spelling pubmed-55739022017-08-30 Single-Molecule Plasmon Sensing: Current Status and Future Prospects Taylor, Adam B. Zijlstra, Peter ACS Sens [Image: see text] Single-molecule detection has long relied on fluorescent labeling with high quantum-yield fluorophores. Plasmon-enhanced detection circumvents the need for labeling by allowing direct optical detection of weakly emitting and completely nonfluorescent species. This review focuses on recent advances in single molecule detection using plasmonic metal nanostructures as a sensing platform, particularly using a single particle–single molecule approach. In the past decade two mechanisms for plasmon-enhanced single-molecule detection have been demonstrated: (1) by plasmonically enhancing the emission of weakly fluorescent biomolecules, or (2) by monitoring shifts of the plasmon resonance induced by single-molecule interactions. We begin with a motivation regarding the importance of single molecule detection, and advantages plasmonic detection offers. We describe both detection mechanisms and discuss challenges and potential solutions. We finalize by highlighting the exciting possibilities in analytical chemistry and medical diagnostics. American Chemical Society 2017-08-01 2017-08-25 /pmc/articles/PMC5573902/ /pubmed/28762723 http://dx.doi.org/10.1021/acssensors.7b00382 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Taylor, Adam B.
Zijlstra, Peter
Single-Molecule Plasmon Sensing: Current Status and Future Prospects
title Single-Molecule Plasmon Sensing: Current Status and Future Prospects
title_full Single-Molecule Plasmon Sensing: Current Status and Future Prospects
title_fullStr Single-Molecule Plasmon Sensing: Current Status and Future Prospects
title_full_unstemmed Single-Molecule Plasmon Sensing: Current Status and Future Prospects
title_short Single-Molecule Plasmon Sensing: Current Status and Future Prospects
title_sort single-molecule plasmon sensing: current status and future prospects
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573902/
https://www.ncbi.nlm.nih.gov/pubmed/28762723
http://dx.doi.org/10.1021/acssensors.7b00382
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