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Single-Molecule Plasmon Sensing: Current Status and Future Prospects
[Image: see text] Single-molecule detection has long relied on fluorescent labeling with high quantum-yield fluorophores. Plasmon-enhanced detection circumvents the need for labeling by allowing direct optical detection of weakly emitting and completely nonfluorescent species. This review focuses on...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573902/ https://www.ncbi.nlm.nih.gov/pubmed/28762723 http://dx.doi.org/10.1021/acssensors.7b00382 |
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author | Taylor, Adam B. Zijlstra, Peter |
author_facet | Taylor, Adam B. Zijlstra, Peter |
author_sort | Taylor, Adam B. |
collection | PubMed |
description | [Image: see text] Single-molecule detection has long relied on fluorescent labeling with high quantum-yield fluorophores. Plasmon-enhanced detection circumvents the need for labeling by allowing direct optical detection of weakly emitting and completely nonfluorescent species. This review focuses on recent advances in single molecule detection using plasmonic metal nanostructures as a sensing platform, particularly using a single particle–single molecule approach. In the past decade two mechanisms for plasmon-enhanced single-molecule detection have been demonstrated: (1) by plasmonically enhancing the emission of weakly fluorescent biomolecules, or (2) by monitoring shifts of the plasmon resonance induced by single-molecule interactions. We begin with a motivation regarding the importance of single molecule detection, and advantages plasmonic detection offers. We describe both detection mechanisms and discuss challenges and potential solutions. We finalize by highlighting the exciting possibilities in analytical chemistry and medical diagnostics. |
format | Online Article Text |
id | pubmed-5573902 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-55739022017-08-30 Single-Molecule Plasmon Sensing: Current Status and Future Prospects Taylor, Adam B. Zijlstra, Peter ACS Sens [Image: see text] Single-molecule detection has long relied on fluorescent labeling with high quantum-yield fluorophores. Plasmon-enhanced detection circumvents the need for labeling by allowing direct optical detection of weakly emitting and completely nonfluorescent species. This review focuses on recent advances in single molecule detection using plasmonic metal nanostructures as a sensing platform, particularly using a single particle–single molecule approach. In the past decade two mechanisms for plasmon-enhanced single-molecule detection have been demonstrated: (1) by plasmonically enhancing the emission of weakly fluorescent biomolecules, or (2) by monitoring shifts of the plasmon resonance induced by single-molecule interactions. We begin with a motivation regarding the importance of single molecule detection, and advantages plasmonic detection offers. We describe both detection mechanisms and discuss challenges and potential solutions. We finalize by highlighting the exciting possibilities in analytical chemistry and medical diagnostics. American Chemical Society 2017-08-01 2017-08-25 /pmc/articles/PMC5573902/ /pubmed/28762723 http://dx.doi.org/10.1021/acssensors.7b00382 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Taylor, Adam B. Zijlstra, Peter Single-Molecule Plasmon Sensing: Current Status and Future Prospects |
title | Single-Molecule Plasmon Sensing: Current Status and
Future Prospects |
title_full | Single-Molecule Plasmon Sensing: Current Status and
Future Prospects |
title_fullStr | Single-Molecule Plasmon Sensing: Current Status and
Future Prospects |
title_full_unstemmed | Single-Molecule Plasmon Sensing: Current Status and
Future Prospects |
title_short | Single-Molecule Plasmon Sensing: Current Status and
Future Prospects |
title_sort | single-molecule plasmon sensing: current status and
future prospects |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573902/ https://www.ncbi.nlm.nih.gov/pubmed/28762723 http://dx.doi.org/10.1021/acssensors.7b00382 |
work_keys_str_mv | AT tayloradamb singlemoleculeplasmonsensingcurrentstatusandfutureprospects AT zijlstrapeter singlemoleculeplasmonsensingcurrentstatusandfutureprospects |