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Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco
BACKGROUND: Bovine tuberculosis (BTB) is caused by Mycobacterium bovis, which belongs to the Mycobacterium tuberculosis complex. Mycobacterium bovis have been described to be responsible of most cases of bovine tuberculosis. Although M. tuberculosis, M. africanum and non-complex mycobacteria were is...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5574129/ https://www.ncbi.nlm.nih.gov/pubmed/28841870 http://dx.doi.org/10.1186/s12917-017-1165-6 |
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author | Yahyaoui-Azami, Hind Aboukhassib, Hamid Bouslikhane, Mohammed Berrada, Jaouad Rami, Soukaina Reinhard, Miriam Gagneux, Sebastien Feldmann, Julia Borrell, Sonia Zinsstag, Jakob |
author_facet | Yahyaoui-Azami, Hind Aboukhassib, Hamid Bouslikhane, Mohammed Berrada, Jaouad Rami, Soukaina Reinhard, Miriam Gagneux, Sebastien Feldmann, Julia Borrell, Sonia Zinsstag, Jakob |
author_sort | Yahyaoui-Azami, Hind |
collection | PubMed |
description | BACKGROUND: Bovine tuberculosis (BTB) is caused by Mycobacterium bovis, which belongs to the Mycobacterium tuberculosis complex. Mycobacterium bovis have been described to be responsible of most cases of bovine tuberculosis. Although M. tuberculosis, M. africanum and non-complex mycobacteria were isolated from cattle. In Morocco, so far, no molecular studies were conducted to characterize the strains responsible of BTB. The present study aims to characterize M. bovis in Morocco. The present study was conducted in slaughterhouses in Rabat and El Jadida. Samples were collected from 327 slaughtered animals with visible lesions suggesting BTB. RESULTS: A total of 225 isolates yielded cultures, 95% (n = 215) of them were acid-fast (AF). Sixty eight per cent of the AF positive samples were confirmed as tuberculous mycobacteria (n = 147), 99% of these (n = 146) having RD9 and among the latter, 98% (n = 143) positive while 2% (n = 3) negative for RD4 A total of 134 samples were analyzed by spoligotyping of which 14 were in cluster and with 41 different spoligotypes, ten of them were new patterns (23%). The most prevalent spoligotypes were SB0121, SB0265, and SB0120, and were already identified in many other countries, such as Algeria, Spain, Tunisia, the United States and Argentina. CONCLUSION: The shared borders between Algeria and Morocco, in addition to the previous importation of cattle from Europe and the US could explain the similarities found in M. bovis spoligotypes. On the other hand, the desert of Morocco could be considered as an efficient barrier preventing the introduction of BTB to Morocco from West Central and East Africa. Our findings suggest a low level endemic transmission of BTB similar to other African countries. However, more research is needed for further knowledge about the transmission patterns of BTB in Morocco. |
format | Online Article Text |
id | pubmed-5574129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55741292017-08-30 Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco Yahyaoui-Azami, Hind Aboukhassib, Hamid Bouslikhane, Mohammed Berrada, Jaouad Rami, Soukaina Reinhard, Miriam Gagneux, Sebastien Feldmann, Julia Borrell, Sonia Zinsstag, Jakob BMC Vet Res Research Article BACKGROUND: Bovine tuberculosis (BTB) is caused by Mycobacterium bovis, which belongs to the Mycobacterium tuberculosis complex. Mycobacterium bovis have been described to be responsible of most cases of bovine tuberculosis. Although M. tuberculosis, M. africanum and non-complex mycobacteria were isolated from cattle. In Morocco, so far, no molecular studies were conducted to characterize the strains responsible of BTB. The present study aims to characterize M. bovis in Morocco. The present study was conducted in slaughterhouses in Rabat and El Jadida. Samples were collected from 327 slaughtered animals with visible lesions suggesting BTB. RESULTS: A total of 225 isolates yielded cultures, 95% (n = 215) of them were acid-fast (AF). Sixty eight per cent of the AF positive samples were confirmed as tuberculous mycobacteria (n = 147), 99% of these (n = 146) having RD9 and among the latter, 98% (n = 143) positive while 2% (n = 3) negative for RD4 A total of 134 samples were analyzed by spoligotyping of which 14 were in cluster and with 41 different spoligotypes, ten of them were new patterns (23%). The most prevalent spoligotypes were SB0121, SB0265, and SB0120, and were already identified in many other countries, such as Algeria, Spain, Tunisia, the United States and Argentina. CONCLUSION: The shared borders between Algeria and Morocco, in addition to the previous importation of cattle from Europe and the US could explain the similarities found in M. bovis spoligotypes. On the other hand, the desert of Morocco could be considered as an efficient barrier preventing the introduction of BTB to Morocco from West Central and East Africa. Our findings suggest a low level endemic transmission of BTB similar to other African countries. However, more research is needed for further knowledge about the transmission patterns of BTB in Morocco. BioMed Central 2017-08-25 /pmc/articles/PMC5574129/ /pubmed/28841870 http://dx.doi.org/10.1186/s12917-017-1165-6 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Yahyaoui-Azami, Hind Aboukhassib, Hamid Bouslikhane, Mohammed Berrada, Jaouad Rami, Soukaina Reinhard, Miriam Gagneux, Sebastien Feldmann, Julia Borrell, Sonia Zinsstag, Jakob Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco |
title | Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco |
title_full | Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco |
title_fullStr | Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco |
title_full_unstemmed | Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco |
title_short | Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco |
title_sort | molecular characterization of bovine tuberculosis strains in two slaughterhouses in morocco |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5574129/ https://www.ncbi.nlm.nih.gov/pubmed/28841870 http://dx.doi.org/10.1186/s12917-017-1165-6 |
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