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Kinetic modelling: an integrated approach to analyze enzyme activity assays

BACKGROUND: In general, enzyme activity is estimated from spectrophotometric data, by taking the slope of the linear part of the progress curve describing the rate of change in the substrate or product monitored. As long as the substrate concentrations are sufficiently high to saturate the enzyme an...

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Detalles Bibliográficos
Autores principales:	Boeckx, Jelena, Hertog, Maarten, Geeraerd, Annemie, Nicolai, Bart
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5574136/ https://www.ncbi.nlm.nih.gov/pubmed/28855956 http://dx.doi.org/10.1186/s13007-017-0218-y

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