Characterization of Leishmania (L.) infantum chagasi in visceral leishmaniasis associated with hiv co-infection in Northeastern Brazil

Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNeal-Nicoll...

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Detalles Bibliográficos
Autores principales: de Lima, Elaine Cristina Bomfim, Barbosa, Walter Lins, de Brito, Maria Edileuza Felinto, de Melo, Fábio Lopes, Brandão, Sinval Pinto, de Medeiros, Zulma Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2017
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5574623/
https://www.ncbi.nlm.nih.gov/pubmed/28902293
http://dx.doi.org/10.1590/S1678-9946201759048
Descripción
Sumario:Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNeal-Nicolle culture and kDNA PCR of peripheral blood were all positive. Typing methods, multilocus enzyme electrophoresis and ITS1-RFLP PCR of peripheral blood confirmed infection by Leishmania (L.) infantum chagasi . PCR has proved to be safer and more affordable than other characterization methods; ITS1-RFLP PCR can diagnose and type Leishmania spp. in both endemic and non-endemic areas, favoring the prognosis and allowing the appropriate treatment of patients.

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