Cargando…
Comparative RNA-Seq Analysis Uncovers a Complex Regulatory Network for Soybean Cyst Nematode Resistance in Wild Soybean (Glycine soja)
Soybean cyst nematode (SCN) is the most damaging pest of soybean worldwide. The molecular mechanism of SCN resistance remains largely unknown. We conducted a global RNA-seq comparison between a resistant genotype (S54) and a susceptible genotype (S67) of Glycine soja, the wild progenitor of soybean,...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575055/ https://www.ncbi.nlm.nih.gov/pubmed/28852059 http://dx.doi.org/10.1038/s41598-017-09945-0 |
_version_ | 1783259961850068992 |
---|---|
author | Zhang, Hengyou Kjemtrup-Lovelace, Susanne Li, Changbao Luo, Yan Chen, Lars P. Song, Bao-Hua |
author_facet | Zhang, Hengyou Kjemtrup-Lovelace, Susanne Li, Changbao Luo, Yan Chen, Lars P. Song, Bao-Hua |
author_sort | Zhang, Hengyou |
collection | PubMed |
description | Soybean cyst nematode (SCN) is the most damaging pest of soybean worldwide. The molecular mechanism of SCN resistance remains largely unknown. We conducted a global RNA-seq comparison between a resistant genotype (S54) and a susceptible genotype (S67) of Glycine soja, the wild progenitor of soybean, to understand its regulatory network in SCN defense. The number of differentially expressed genes (DEGs) in S54 (2,290) was much larger than that in S67 (555). A number of defense-related genes/pathways were significantly induced only in S54, while photosynthesis and several metabolic pathways were affected in both genotypes with SCN infection. These defense-associated DEGs were involved in pathogen recognition, calcium/calmodulin-mediated defense signaling, jasmonic acid (JA)/ethylene (ET) and sialic acid (SA)-involved signaling, the MAPK signaling cascade, and WRKY-involved transcriptional regulation. Our results revealed a comprehensive regulatory network involved in SCN resistance and provided insights into the complex molecular mechanisms of SCN resistance in wild soybean. |
format | Online Article Text |
id | pubmed-5575055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55750552017-09-01 Comparative RNA-Seq Analysis Uncovers a Complex Regulatory Network for Soybean Cyst Nematode Resistance in Wild Soybean (Glycine soja) Zhang, Hengyou Kjemtrup-Lovelace, Susanne Li, Changbao Luo, Yan Chen, Lars P. Song, Bao-Hua Sci Rep Article Soybean cyst nematode (SCN) is the most damaging pest of soybean worldwide. The molecular mechanism of SCN resistance remains largely unknown. We conducted a global RNA-seq comparison between a resistant genotype (S54) and a susceptible genotype (S67) of Glycine soja, the wild progenitor of soybean, to understand its regulatory network in SCN defense. The number of differentially expressed genes (DEGs) in S54 (2,290) was much larger than that in S67 (555). A number of defense-related genes/pathways were significantly induced only in S54, while photosynthesis and several metabolic pathways were affected in both genotypes with SCN infection. These defense-associated DEGs were involved in pathogen recognition, calcium/calmodulin-mediated defense signaling, jasmonic acid (JA)/ethylene (ET) and sialic acid (SA)-involved signaling, the MAPK signaling cascade, and WRKY-involved transcriptional regulation. Our results revealed a comprehensive regulatory network involved in SCN resistance and provided insights into the complex molecular mechanisms of SCN resistance in wild soybean. Nature Publishing Group UK 2017-08-29 /pmc/articles/PMC5575055/ /pubmed/28852059 http://dx.doi.org/10.1038/s41598-017-09945-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zhang, Hengyou Kjemtrup-Lovelace, Susanne Li, Changbao Luo, Yan Chen, Lars P. Song, Bao-Hua Comparative RNA-Seq Analysis Uncovers a Complex Regulatory Network for Soybean Cyst Nematode Resistance in Wild Soybean (Glycine soja) |
title | Comparative RNA-Seq Analysis Uncovers a Complex Regulatory Network for Soybean Cyst Nematode Resistance in Wild Soybean (Glycine soja) |
title_full | Comparative RNA-Seq Analysis Uncovers a Complex Regulatory Network for Soybean Cyst Nematode Resistance in Wild Soybean (Glycine soja) |
title_fullStr | Comparative RNA-Seq Analysis Uncovers a Complex Regulatory Network for Soybean Cyst Nematode Resistance in Wild Soybean (Glycine soja) |
title_full_unstemmed | Comparative RNA-Seq Analysis Uncovers a Complex Regulatory Network for Soybean Cyst Nematode Resistance in Wild Soybean (Glycine soja) |
title_short | Comparative RNA-Seq Analysis Uncovers a Complex Regulatory Network for Soybean Cyst Nematode Resistance in Wild Soybean (Glycine soja) |
title_sort | comparative rna-seq analysis uncovers a complex regulatory network for soybean cyst nematode resistance in wild soybean (glycine soja) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575055/ https://www.ncbi.nlm.nih.gov/pubmed/28852059 http://dx.doi.org/10.1038/s41598-017-09945-0 |
work_keys_str_mv | AT zhanghengyou comparativernaseqanalysisuncoversacomplexregulatorynetworkforsoybeancystnematoderesistanceinwildsoybeanglycinesoja AT kjemtruplovelacesusanne comparativernaseqanalysisuncoversacomplexregulatorynetworkforsoybeancystnematoderesistanceinwildsoybeanglycinesoja AT lichangbao comparativernaseqanalysisuncoversacomplexregulatorynetworkforsoybeancystnematoderesistanceinwildsoybeanglycinesoja AT luoyan comparativernaseqanalysisuncoversacomplexregulatorynetworkforsoybeancystnematoderesistanceinwildsoybeanglycinesoja AT chenlarsp comparativernaseqanalysisuncoversacomplexregulatorynetworkforsoybeancystnematoderesistanceinwildsoybeanglycinesoja AT songbaohua comparativernaseqanalysisuncoversacomplexregulatorynetworkforsoybeancystnematoderesistanceinwildsoybeanglycinesoja |