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Microtubules Modulate F-actin Dynamics during Neuronal Polarization
Neuronal polarization is reflected by different dynamics of microtubule and filamentous actin (F-actin). Axonal microtubules are more stable than those in the remaining neurites, while dynamics of F-actin in axonal growth cones clearly exceed those in their dendritic counterparts. However, whether a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575062/ https://www.ncbi.nlm.nih.gov/pubmed/28851982 http://dx.doi.org/10.1038/s41598-017-09832-8 |
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author | Zhao, Bing Meka, Durga Praveen Scharrenberg, Robin König, Theresa Schwanke, Birgit Kobler, Oliver Windhorst, Sabine Kreutz, Michael R. Mikhaylova, Marina Calderon de Anda, Froylan |
author_facet | Zhao, Bing Meka, Durga Praveen Scharrenberg, Robin König, Theresa Schwanke, Birgit Kobler, Oliver Windhorst, Sabine Kreutz, Michael R. Mikhaylova, Marina Calderon de Anda, Froylan |
author_sort | Zhao, Bing |
collection | PubMed |
description | Neuronal polarization is reflected by different dynamics of microtubule and filamentous actin (F-actin). Axonal microtubules are more stable than those in the remaining neurites, while dynamics of F-actin in axonal growth cones clearly exceed those in their dendritic counterparts. However, whether a functional interplay exists between the microtubule network and F-actin dynamics in growing axons and whether this interplay is instrumental for breaking cellular symmetry is currently unknown. Here, we show that an increment on microtubule stability or number of microtubules is associated with increased F-actin dynamics. Moreover, we show that Drebrin E, an F-actin and microtubule plus-end binding protein, mediates this cross talk. Drebrin E segregates preferentially to growth cones with a higher F-actin treadmilling rate, where more microtubule plus-ends are found. Interruption of the interaction of Drebrin E with microtubules decreases F-actin dynamics and arrests neuronal polarization. Collectively the data show that microtubules modulate F-actin dynamics for initial axon extension during neuronal development. |
format | Online Article Text |
id | pubmed-5575062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55750622017-09-01 Microtubules Modulate F-actin Dynamics during Neuronal Polarization Zhao, Bing Meka, Durga Praveen Scharrenberg, Robin König, Theresa Schwanke, Birgit Kobler, Oliver Windhorst, Sabine Kreutz, Michael R. Mikhaylova, Marina Calderon de Anda, Froylan Sci Rep Article Neuronal polarization is reflected by different dynamics of microtubule and filamentous actin (F-actin). Axonal microtubules are more stable than those in the remaining neurites, while dynamics of F-actin in axonal growth cones clearly exceed those in their dendritic counterparts. However, whether a functional interplay exists between the microtubule network and F-actin dynamics in growing axons and whether this interplay is instrumental for breaking cellular symmetry is currently unknown. Here, we show that an increment on microtubule stability or number of microtubules is associated with increased F-actin dynamics. Moreover, we show that Drebrin E, an F-actin and microtubule plus-end binding protein, mediates this cross talk. Drebrin E segregates preferentially to growth cones with a higher F-actin treadmilling rate, where more microtubule plus-ends are found. Interruption of the interaction of Drebrin E with microtubules decreases F-actin dynamics and arrests neuronal polarization. Collectively the data show that microtubules modulate F-actin dynamics for initial axon extension during neuronal development. Nature Publishing Group UK 2017-08-29 /pmc/articles/PMC5575062/ /pubmed/28851982 http://dx.doi.org/10.1038/s41598-017-09832-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zhao, Bing Meka, Durga Praveen Scharrenberg, Robin König, Theresa Schwanke, Birgit Kobler, Oliver Windhorst, Sabine Kreutz, Michael R. Mikhaylova, Marina Calderon de Anda, Froylan Microtubules Modulate F-actin Dynamics during Neuronal Polarization |
title | Microtubules Modulate F-actin Dynamics during Neuronal Polarization |
title_full | Microtubules Modulate F-actin Dynamics during Neuronal Polarization |
title_fullStr | Microtubules Modulate F-actin Dynamics during Neuronal Polarization |
title_full_unstemmed | Microtubules Modulate F-actin Dynamics during Neuronal Polarization |
title_short | Microtubules Modulate F-actin Dynamics during Neuronal Polarization |
title_sort | microtubules modulate f-actin dynamics during neuronal polarization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575062/ https://www.ncbi.nlm.nih.gov/pubmed/28851982 http://dx.doi.org/10.1038/s41598-017-09832-8 |
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