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Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis)

Tea plant is a typical fluorine (F) accumulator. F concentration in mature tea leaves is several hundred times higher than that in normal field crops. Long-term consumption of teas with high level F will increase the risks of dental and skeletal fluorosis. The mechanism of F accumulation in tea stan...

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Autores principales: Li, Qing-Sheng, Lin, Xiao-Ming, Qiao, Ru-Ying, Zheng, Xin-Qiang, Lu, Jian-Liang, Ye, Jian-Hui, Liang, Yue-Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575122/
https://www.ncbi.nlm.nih.gov/pubmed/28851890
http://dx.doi.org/10.1038/s41598-017-08587-6
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author Li, Qing-Sheng
Lin, Xiao-Ming
Qiao, Ru-Ying
Zheng, Xin-Qiang
Lu, Jian-Liang
Ye, Jian-Hui
Liang, Yue-Rong
author_facet Li, Qing-Sheng
Lin, Xiao-Ming
Qiao, Ru-Ying
Zheng, Xin-Qiang
Lu, Jian-Liang
Ye, Jian-Hui
Liang, Yue-Rong
author_sort Li, Qing-Sheng
collection PubMed
description Tea plant is a typical fluorine (F) accumulator. F concentration in mature tea leaves is several hundred times higher than that in normal field crops. Long-term consumption of teas with high level F will increase the risks of dental and skeletal fluorosis. The mechanism of F accumulation in tea stands unclear. RNA-Seq and digital gene expression (DGE) techniques were used to investigate the effect of F on the differential expressions of transcriptome in tea plant. The results showed that F content in mature tea leaves was increased with increase in F concentration of cultural solution and duration of F treatment time. Based on comparison with data of GO, COG, KEGG and Nr databases, 144 differentially expressed unigenes with definite annotation were identified. Real-time reverse transcription PCR (qRT-PCR) was used to validate the effect of F on expression of 5 unigenes screened from the 144 unigenes. F treatment induced the expression of defense genes such as receptor-like kinases (RLKs) and U-box domain-containing protein. Based on the present study, F uptake is considered to be related to calcium-transporting ATPase, especially autoinhibited Ca(2+) ATPase (ACAs) which was activated by the RLKs and worked as a carrier in uptake of F by tea plant.
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spelling pubmed-55751222017-09-01 Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis) Li, Qing-Sheng Lin, Xiao-Ming Qiao, Ru-Ying Zheng, Xin-Qiang Lu, Jian-Liang Ye, Jian-Hui Liang, Yue-Rong Sci Rep Article Tea plant is a typical fluorine (F) accumulator. F concentration in mature tea leaves is several hundred times higher than that in normal field crops. Long-term consumption of teas with high level F will increase the risks of dental and skeletal fluorosis. The mechanism of F accumulation in tea stands unclear. RNA-Seq and digital gene expression (DGE) techniques were used to investigate the effect of F on the differential expressions of transcriptome in tea plant. The results showed that F content in mature tea leaves was increased with increase in F concentration of cultural solution and duration of F treatment time. Based on comparison with data of GO, COG, KEGG and Nr databases, 144 differentially expressed unigenes with definite annotation were identified. Real-time reverse transcription PCR (qRT-PCR) was used to validate the effect of F on expression of 5 unigenes screened from the 144 unigenes. F treatment induced the expression of defense genes such as receptor-like kinases (RLKs) and U-box domain-containing protein. Based on the present study, F uptake is considered to be related to calcium-transporting ATPase, especially autoinhibited Ca(2+) ATPase (ACAs) which was activated by the RLKs and worked as a carrier in uptake of F by tea plant. Nature Publishing Group UK 2017-08-29 /pmc/articles/PMC5575122/ /pubmed/28851890 http://dx.doi.org/10.1038/s41598-017-08587-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Qing-Sheng
Lin, Xiao-Ming
Qiao, Ru-Ying
Zheng, Xin-Qiang
Lu, Jian-Liang
Ye, Jian-Hui
Liang, Yue-Rong
Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis)
title Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis)
title_full Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis)
title_fullStr Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis)
title_full_unstemmed Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis)
title_short Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis)
title_sort effect of fluoride treatment on gene expression in tea plant (camellia sinensis)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575122/
https://www.ncbi.nlm.nih.gov/pubmed/28851890
http://dx.doi.org/10.1038/s41598-017-08587-6
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