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Fast free-of-acrylamide clearing tissue (FACT)—an optimized new protocol for rapid, high-resolution imaging of three-dimensional brain tissue

Fast Free-of-Acrylamide Clearing Tissue (FACT) is a new sodium dodecyl sulfate (SDS)-based clearing protocol for the chemical clearing and imaging of brain tissue containing transgenic or immunolabeled fluorescent proteins. In the present study, we have developed this new method and optimized multip...

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Detalles Bibliográficos
Autores principales: Xu, Na, Tamadon, Amin, Liu, Yaan, Ma, Tong, Leak, Rehana K., Chen, Jun, Gao, Yanqin, Feng, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575133/
https://www.ncbi.nlm.nih.gov/pubmed/28852046
http://dx.doi.org/10.1038/s41598-017-10204-5