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Engineering of versatile redox partner fusions that support monooxygenase activity of functionally diverse cytochrome P450s

Most bacterial cytochrome P450 monooxygenases (P450s or CYPs) require two redox partner proteins for activity. To reduce complexity of the redox chain, the Bacillus subtilis flavodoxin YkuN (Y) was fused to the Escherichia coli flavodoxin reductase Fpr (R), and activity was tuned by placing flexible...

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Detalles Bibliográficos
Autores principales:	Bakkes, Patrick J., Riehm, Jan L., Sagadin, Tanja, Rühlmann, Ansgar, Schubert, Peter, Biemann, Stefan, Girhard, Marco, Hutter, Michael C., Bernhardt, Rita, Urlacher, Vlada B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575160/ https://www.ncbi.nlm.nih.gov/pubmed/28852040 http://dx.doi.org/10.1038/s41598-017-10075-w

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