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Mapping architectural and transcriptional alterations in non-lesional and lesional epidermis in vitiligo

In vitiligo, chronic loss of melanocytes and consequent absence of melanin from the epidermis presents a challenge for long-term tissue maintenance. The stable vitiligo patches are known to attain an irreversible depigmented state. However, the molecular and cellular processes resulting in this remo...

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Autores principales: Singh, Archana, Gotherwal, Vishvabandhu, Junni, Päivi, Vijayan, Vinaya, Tiwari, Manisha, Ganju, Parul, Kumar, Avinash, Sharma, Pankaj, Fatima, Tanveer, Gupta, Aayush, Holla, Ananthaprasad, Kar, Hemanta K., Khanna, Sangeeta, Thukral, Lipi, Malik, Garima, Natarajan, Krishnamurthy, Gadgil, Chetan J., Lahesmaa, Riitta, Natarajan, Vivek T., Rani, Rajni, Gokhale, Rajesh S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575244/
https://www.ncbi.nlm.nih.gov/pubmed/28852211
http://dx.doi.org/10.1038/s41598-017-10253-w
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author Singh, Archana
Gotherwal, Vishvabandhu
Junni, Päivi
Vijayan, Vinaya
Tiwari, Manisha
Ganju, Parul
Kumar, Avinash
Sharma, Pankaj
Fatima, Tanveer
Gupta, Aayush
Holla, Ananthaprasad
Kar, Hemanta K.
Khanna, Sangeeta
Thukral, Lipi
Malik, Garima
Natarajan, Krishnamurthy
Gadgil, Chetan J.
Lahesmaa, Riitta
Natarajan, Vivek T.
Rani, Rajni
Gokhale, Rajesh S.
author_facet Singh, Archana
Gotherwal, Vishvabandhu
Junni, Päivi
Vijayan, Vinaya
Tiwari, Manisha
Ganju, Parul
Kumar, Avinash
Sharma, Pankaj
Fatima, Tanveer
Gupta, Aayush
Holla, Ananthaprasad
Kar, Hemanta K.
Khanna, Sangeeta
Thukral, Lipi
Malik, Garima
Natarajan, Krishnamurthy
Gadgil, Chetan J.
Lahesmaa, Riitta
Natarajan, Vivek T.
Rani, Rajni
Gokhale, Rajesh S.
author_sort Singh, Archana
collection PubMed
description In vitiligo, chronic loss of melanocytes and consequent absence of melanin from the epidermis presents a challenge for long-term tissue maintenance. The stable vitiligo patches are known to attain an irreversible depigmented state. However, the molecular and cellular processes resulting in this remodeled tissue homeostasis is unclear. To investigate the complex interplay of inductive signals and cell intrinsic factors that support the new acquired state, we compared the matched lesional and non-lesional epidermis obtained from stable non-segmental vitiligo subjects. Hierarchical clustering of genome-wide expression of transcripts surprisingly segregated lesional and non-lesional samples in two distinct clades, despite the apparent heterogeneity in the lesions of different vitiligo subjects. Pathway enrichment showed the expected downregulation of melanogenic pathway and a significant downregulation of cornification and keratinocyte differentiation processes. These perturbations could indeed be recapitulated in the lesional epidermal tissue, including blunting of rete-ridges, thickening of stratum corneum and increase in the size of corneocytes. In addition, we identify marked increase in the putrescine levels due to the elevated expression of spermine/spermidine acetyl transferase. Our study provides insights into the intrinsic self-renewing ability of damaged lesional tissue to restore epidermal functionality in vitiligo.
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spelling pubmed-55752442017-09-01 Mapping architectural and transcriptional alterations in non-lesional and lesional epidermis in vitiligo Singh, Archana Gotherwal, Vishvabandhu Junni, Päivi Vijayan, Vinaya Tiwari, Manisha Ganju, Parul Kumar, Avinash Sharma, Pankaj Fatima, Tanveer Gupta, Aayush Holla, Ananthaprasad Kar, Hemanta K. Khanna, Sangeeta Thukral, Lipi Malik, Garima Natarajan, Krishnamurthy Gadgil, Chetan J. Lahesmaa, Riitta Natarajan, Vivek T. Rani, Rajni Gokhale, Rajesh S. Sci Rep Article In vitiligo, chronic loss of melanocytes and consequent absence of melanin from the epidermis presents a challenge for long-term tissue maintenance. The stable vitiligo patches are known to attain an irreversible depigmented state. However, the molecular and cellular processes resulting in this remodeled tissue homeostasis is unclear. To investigate the complex interplay of inductive signals and cell intrinsic factors that support the new acquired state, we compared the matched lesional and non-lesional epidermis obtained from stable non-segmental vitiligo subjects. Hierarchical clustering of genome-wide expression of transcripts surprisingly segregated lesional and non-lesional samples in two distinct clades, despite the apparent heterogeneity in the lesions of different vitiligo subjects. Pathway enrichment showed the expected downregulation of melanogenic pathway and a significant downregulation of cornification and keratinocyte differentiation processes. These perturbations could indeed be recapitulated in the lesional epidermal tissue, including blunting of rete-ridges, thickening of stratum corneum and increase in the size of corneocytes. In addition, we identify marked increase in the putrescine levels due to the elevated expression of spermine/spermidine acetyl transferase. Our study provides insights into the intrinsic self-renewing ability of damaged lesional tissue to restore epidermal functionality in vitiligo. Nature Publishing Group UK 2017-08-29 /pmc/articles/PMC5575244/ /pubmed/28852211 http://dx.doi.org/10.1038/s41598-017-10253-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Singh, Archana
Gotherwal, Vishvabandhu
Junni, Päivi
Vijayan, Vinaya
Tiwari, Manisha
Ganju, Parul
Kumar, Avinash
Sharma, Pankaj
Fatima, Tanveer
Gupta, Aayush
Holla, Ananthaprasad
Kar, Hemanta K.
Khanna, Sangeeta
Thukral, Lipi
Malik, Garima
Natarajan, Krishnamurthy
Gadgil, Chetan J.
Lahesmaa, Riitta
Natarajan, Vivek T.
Rani, Rajni
Gokhale, Rajesh S.
Mapping architectural and transcriptional alterations in non-lesional and lesional epidermis in vitiligo
title Mapping architectural and transcriptional alterations in non-lesional and lesional epidermis in vitiligo
title_full Mapping architectural and transcriptional alterations in non-lesional and lesional epidermis in vitiligo
title_fullStr Mapping architectural and transcriptional alterations in non-lesional and lesional epidermis in vitiligo
title_full_unstemmed Mapping architectural and transcriptional alterations in non-lesional and lesional epidermis in vitiligo
title_short Mapping architectural and transcriptional alterations in non-lesional and lesional epidermis in vitiligo
title_sort mapping architectural and transcriptional alterations in non-lesional and lesional epidermis in vitiligo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575244/
https://www.ncbi.nlm.nih.gov/pubmed/28852211
http://dx.doi.org/10.1038/s41598-017-10253-w
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