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Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells
Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation and characterisation procedures. This...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575260/ https://www.ncbi.nlm.nih.gov/pubmed/28852131 http://dx.doi.org/10.1038/s41598-017-10448-1 |
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author | Gualerzi, Alice Niada, Stefania Giannasi, Chiara Picciolini, Silvia Morasso, Carlo Vanna, Renzo Rossella, Valeria Masserini, Massimo Bedoni, Marzia Ciceri, Fabio Bernardo, Maria Ester Brini, Anna Teresa Gramatica, Furio |
author_facet | Gualerzi, Alice Niada, Stefania Giannasi, Chiara Picciolini, Silvia Morasso, Carlo Vanna, Renzo Rossella, Valeria Masserini, Massimo Bedoni, Marzia Ciceri, Fabio Bernardo, Maria Ester Brini, Anna Teresa Gramatica, Furio |
author_sort | Gualerzi, Alice |
collection | PubMed |
description | Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation and characterisation procedures. This study shows for the first time the successful application of Raman spectroscopy as label-free, sensitive and reproducible means of carrying out the routine bulk characterisation of MSC-derived vesicles before their use in vitro or in vivo, thus promoting the translation of EV research to clinical practice. The Raman spectra of the EVs of bone marrow and adipose tissue-derived MSCs were compared with human dermal fibroblast EVs in order to demonstrate the ability of the method to distinguish the vesicles of the three cytotypes automatically with an accuracy of 93.7%. Our data attribute a Raman fingerprint to EVs from undifferentiated and differentiated cells of diverse tissue origin, and provide insights into the biochemical characteristics of EVs from different sources and into the differential contribution of sphingomyelin, gangliosides and phosphatidilcholine to the Raman spectra themselves. |
format | Online Article Text |
id | pubmed-5575260 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55752602017-09-01 Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells Gualerzi, Alice Niada, Stefania Giannasi, Chiara Picciolini, Silvia Morasso, Carlo Vanna, Renzo Rossella, Valeria Masserini, Massimo Bedoni, Marzia Ciceri, Fabio Bernardo, Maria Ester Brini, Anna Teresa Gramatica, Furio Sci Rep Article Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation and characterisation procedures. This study shows for the first time the successful application of Raman spectroscopy as label-free, sensitive and reproducible means of carrying out the routine bulk characterisation of MSC-derived vesicles before their use in vitro or in vivo, thus promoting the translation of EV research to clinical practice. The Raman spectra of the EVs of bone marrow and adipose tissue-derived MSCs were compared with human dermal fibroblast EVs in order to demonstrate the ability of the method to distinguish the vesicles of the three cytotypes automatically with an accuracy of 93.7%. Our data attribute a Raman fingerprint to EVs from undifferentiated and differentiated cells of diverse tissue origin, and provide insights into the biochemical characteristics of EVs from different sources and into the differential contribution of sphingomyelin, gangliosides and phosphatidilcholine to the Raman spectra themselves. Nature Publishing Group UK 2017-08-29 /pmc/articles/PMC5575260/ /pubmed/28852131 http://dx.doi.org/10.1038/s41598-017-10448-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gualerzi, Alice Niada, Stefania Giannasi, Chiara Picciolini, Silvia Morasso, Carlo Vanna, Renzo Rossella, Valeria Masserini, Massimo Bedoni, Marzia Ciceri, Fabio Bernardo, Maria Ester Brini, Anna Teresa Gramatica, Furio Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells |
title | Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells |
title_full | Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells |
title_fullStr | Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells |
title_full_unstemmed | Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells |
title_short | Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells |
title_sort | raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575260/ https://www.ncbi.nlm.nih.gov/pubmed/28852131 http://dx.doi.org/10.1038/s41598-017-10448-1 |
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