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Improved split fluorescent proteins for endogenous protein labeling
Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell–cell contact. To expand this toolset, we have developed a screening strategy for the direct engineering of self-complementing split...
Autores principales: | Feng, Siyu, Sekine, Sayaka, Pessino, Veronica, Li, Han, Leonetti, Manuel D., Huang, Bo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575300/ https://www.ncbi.nlm.nih.gov/pubmed/28851864 http://dx.doi.org/10.1038/s41467-017-00494-8 |
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