Assessing the Influence of Mutation on GTPase Transition States by Using X‐ray Crystallography, (19)F NMR, and DFT Approaches

We report X‐ray crystallographic and (19)F NMR studies of the G‐protein RhoA complexed with MgF(3) (−), GDP, and RhoGAP, which has the mutation Arg85′Ala. When combined with DFT calculations, these data permit the identification of changes in transition state (TS) properties. The X‐ray data show how...

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Detalles Bibliográficos
Autores principales: Jin, Yi, Molt, Robert W., Pellegrini, Erika, Cliff, Matthew J., Bowler, Matthew W., Richards, Nigel G. J., Blackburn, G. Michael, Waltho, Jonathan P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575484/
https://www.ncbi.nlm.nih.gov/pubmed/28498638
http://dx.doi.org/10.1002/anie.201703074

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