Cross‐Regulation of an Artificial Metalloenzyme

Cross‐regulation of complex biochemical reaction networks is an essential feature of living systems. In a biomimetic spirit, we report on our efforts to program the temporal activation of an artificial metalloenzyme via cross‐regulation by a natural enzyme. In the presence of urea, urease slowly rel...

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Detalles Bibliográficos
Autores principales: Okamoto, Yasunori, Ward, Thomas R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575532/
https://www.ncbi.nlm.nih.gov/pubmed/28485105
http://dx.doi.org/10.1002/anie.201702181
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author Okamoto, Yasunori
Ward, Thomas R.
author_facet Okamoto, Yasunori
Ward, Thomas R.
author_sort Okamoto, Yasunori
collection PubMed
description Cross‐regulation of complex biochemical reaction networks is an essential feature of living systems. In a biomimetic spirit, we report on our efforts to program the temporal activation of an artificial metalloenzyme via cross‐regulation by a natural enzyme. In the presence of urea, urease slowly releases ammonia that reversibly inhibits an artificial transfer hydrogenase. Addition of an acid, which acts as fuel, allows to maintain the system out of equilibrium.
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spelling pubmed-55755322017-09-18 Cross‐Regulation of an Artificial Metalloenzyme Okamoto, Yasunori Ward, Thomas R. Angew Chem Int Ed Engl Communications Cross‐regulation of complex biochemical reaction networks is an essential feature of living systems. In a biomimetic spirit, we report on our efforts to program the temporal activation of an artificial metalloenzyme via cross‐regulation by a natural enzyme. In the presence of urea, urease slowly releases ammonia that reversibly inhibits an artificial transfer hydrogenase. Addition of an acid, which acts as fuel, allows to maintain the system out of equilibrium. John Wiley and Sons Inc. 2017-05-31 2017-08-14 /pmc/articles/PMC5575532/ /pubmed/28485105 http://dx.doi.org/10.1002/anie.201702181 Text en © 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Communications
Okamoto, Yasunori
Ward, Thomas R.
Cross‐Regulation of an Artificial Metalloenzyme
title Cross‐Regulation of an Artificial Metalloenzyme
title_full Cross‐Regulation of an Artificial Metalloenzyme
title_fullStr Cross‐Regulation of an Artificial Metalloenzyme
title_full_unstemmed Cross‐Regulation of an Artificial Metalloenzyme
title_short Cross‐Regulation of an Artificial Metalloenzyme
title_sort cross‐regulation of an artificial metalloenzyme
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575532/
https://www.ncbi.nlm.nih.gov/pubmed/28485105
http://dx.doi.org/10.1002/anie.201702181
work_keys_str_mv AT okamotoyasunori crossregulationofanartificialmetalloenzyme
AT wardthomasr crossregulationofanartificialmetalloenzyme

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