Impact of Larger Sputum Volume on Xpert(®) MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis

As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5–10 mL) were tested with Xpert(®) MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this st...

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Autores principales: Badal-Faesen, Sharlaa, Firnhaber, Cynthia, Kendall, Michelle A., Wu, Xingye, Grinsztejn, Beatriz, Escada, Rodrigo Otavio da Silva, Fernandez, Michel, Hogg, Evelyn, Sanne, Ian, Johnson, Pamela, Alland, David, Mazurek, Gerald H., Benator, Debra A., Luetkemeyer, Anne F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575580/
https://www.ncbi.nlm.nih.gov/pubmed/28783093
http://dx.doi.org/10.3390/jcm6080078
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author Badal-Faesen, Sharlaa
Firnhaber, Cynthia
Kendall, Michelle A.
Wu, Xingye
Grinsztejn, Beatriz
Escada, Rodrigo Otavio da Silva
Fernandez, Michel
Hogg, Evelyn
Sanne, Ian
Johnson, Pamela
Alland, David
Mazurek, Gerald H.
Benator, Debra A.
Luetkemeyer, Anne F.
author_facet Badal-Faesen, Sharlaa
Firnhaber, Cynthia
Kendall, Michelle A.
Wu, Xingye
Grinsztejn, Beatriz
Escada, Rodrigo Otavio da Silva
Fernandez, Michel
Hogg, Evelyn
Sanne, Ian
Johnson, Pamela
Alland, David
Mazurek, Gerald H.
Benator, Debra A.
Luetkemeyer, Anne F.
author_sort Badal-Faesen, Sharlaa
collection PubMed
description As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5–10 mL) were tested with Xpert(®) MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this strategy had a suboptimal sensitivity of 50% (4/8).
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spelling pubmed-55755802017-09-01 Impact of Larger Sputum Volume on Xpert(®) MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis Badal-Faesen, Sharlaa Firnhaber, Cynthia Kendall, Michelle A. Wu, Xingye Grinsztejn, Beatriz Escada, Rodrigo Otavio da Silva Fernandez, Michel Hogg, Evelyn Sanne, Ian Johnson, Pamela Alland, David Mazurek, Gerald H. Benator, Debra A. Luetkemeyer, Anne F. J Clin Med Brief Report As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5–10 mL) were tested with Xpert(®) MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this strategy had a suboptimal sensitivity of 50% (4/8). MDPI 2017-08-07 /pmc/articles/PMC5575580/ /pubmed/28783093 http://dx.doi.org/10.3390/jcm6080078 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Badal-Faesen, Sharlaa
Firnhaber, Cynthia
Kendall, Michelle A.
Wu, Xingye
Grinsztejn, Beatriz
Escada, Rodrigo Otavio da Silva
Fernandez, Michel
Hogg, Evelyn
Sanne, Ian
Johnson, Pamela
Alland, David
Mazurek, Gerald H.
Benator, Debra A.
Luetkemeyer, Anne F.
Impact of Larger Sputum Volume on Xpert(®) MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
title Impact of Larger Sputum Volume on Xpert(®) MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
title_full Impact of Larger Sputum Volume on Xpert(®) MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
title_fullStr Impact of Larger Sputum Volume on Xpert(®) MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
title_full_unstemmed Impact of Larger Sputum Volume on Xpert(®) MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
title_short Impact of Larger Sputum Volume on Xpert(®) MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
title_sort impact of larger sputum volume on xpert(®) mtb/rif assay detection of mycobacterium tuberculosis in smear-negative individuals with suspected tuberculosis
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575580/
https://www.ncbi.nlm.nih.gov/pubmed/28783093
http://dx.doi.org/10.3390/jcm6080078
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