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Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis

Most of the eukaryotic genes contain introns, which are removed from the pre-RNA during RNA processing. In contrast to the introns in animals, which are usually several kilo base pairs (kb), those in plants generally are very small, which are mostly from dozens of base pairs (bp) to a few hundred bp...

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Autores principales: Chang, Ning, Sun, Qingqing, Hu, Jinglei, An, Chuanjing, Gao, Hongbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575664/
https://www.ncbi.nlm.nih.gov/pubmed/28800125
http://dx.doi.org/10.3390/genes8080200
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author Chang, Ning
Sun, Qingqing
Hu, Jinglei
An, Chuanjing
Gao, Hongbo
author_facet Chang, Ning
Sun, Qingqing
Hu, Jinglei
An, Chuanjing
Gao, Hongbo
author_sort Chang, Ning
collection PubMed
description Most of the eukaryotic genes contain introns, which are removed from the pre-RNA during RNA processing. In contrast to the introns in animals, which are usually several kilo base pairs (kb), those in plants generally are very small, which are mostly from dozens of base pairs (bp) to a few hundred bp. According to annotation version 10.0 of the genome of Arabidopsis thaliana, there are 127,854 introns in the nuclear genes; 99.23% of them are less than 1 kb, and only 16 introns are annotated to be larger than 5 kb, which are extremely large introns (ELI) in Arabidopsis. To learn whether these introns are true introns or not and how large introns could be in Arabidopsis, RT-PCR analysis of genes containing these ELIs were carried out. The results indicated that some of these putative introns are indeed ELIs. These ELIs are mainly composed of transposons or transposable elements (TE), excepting one, whose counterparts are also very long in diverse plant species. Thus, this study confirms the existence of introns larger than 5 kb or even 10 kb in Arabidopsis.
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spelling pubmed-55756642017-09-01 Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis Chang, Ning Sun, Qingqing Hu, Jinglei An, Chuanjing Gao, Hongbo Genes (Basel) Article Most of the eukaryotic genes contain introns, which are removed from the pre-RNA during RNA processing. In contrast to the introns in animals, which are usually several kilo base pairs (kb), those in plants generally are very small, which are mostly from dozens of base pairs (bp) to a few hundred bp. According to annotation version 10.0 of the genome of Arabidopsis thaliana, there are 127,854 introns in the nuclear genes; 99.23% of them are less than 1 kb, and only 16 introns are annotated to be larger than 5 kb, which are extremely large introns (ELI) in Arabidopsis. To learn whether these introns are true introns or not and how large introns could be in Arabidopsis, RT-PCR analysis of genes containing these ELIs were carried out. The results indicated that some of these putative introns are indeed ELIs. These ELIs are mainly composed of transposons or transposable elements (TE), excepting one, whose counterparts are also very long in diverse plant species. Thus, this study confirms the existence of introns larger than 5 kb or even 10 kb in Arabidopsis. MDPI 2017-08-11 /pmc/articles/PMC5575664/ /pubmed/28800125 http://dx.doi.org/10.3390/genes8080200 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chang, Ning
Sun, Qingqing
Hu, Jinglei
An, Chuanjing
Gao, Hongbo
Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis
title Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis
title_full Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis
title_fullStr Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis
title_full_unstemmed Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis
title_short Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis
title_sort large introns of 5 to 10 kilo base pairs can be spliced out in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575664/
https://www.ncbi.nlm.nih.gov/pubmed/28800125
http://dx.doi.org/10.3390/genes8080200
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