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The protein phosphatase 4 complex promotes the Notch pathway and wingless transcription
The Wnt/Wingless (Wg) pathway controls cell fate specification, tissue differentiation and organ development across organisms. Using an in vivo RNAi screen to identify novel kinase and phosphatase regulators of the Wg pathway, we identified subunits of the serine threonine phosphatase Protein Phosph...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576076/ https://www.ncbi.nlm.nih.gov/pubmed/28652317 http://dx.doi.org/10.1242/bio.025221 |
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author | Hall, Eric T. Pradhan-Sundd, Tirthadipa Samnani, Faaria Verheyen, Esther M. |
author_facet | Hall, Eric T. Pradhan-Sundd, Tirthadipa Samnani, Faaria Verheyen, Esther M. |
author_sort | Hall, Eric T. |
collection | PubMed |
description | The Wnt/Wingless (Wg) pathway controls cell fate specification, tissue differentiation and organ development across organisms. Using an in vivo RNAi screen to identify novel kinase and phosphatase regulators of the Wg pathway, we identified subunits of the serine threonine phosphatase Protein Phosphatase 4 (PP4). Knockdown of the catalytic and regulatory subunits of PP4 cause reductions in the Wg pathway targets Senseless and Distal-less. We find that PP4 regulates the Wg pathway by controlling Notch-driven wg transcription. Genetic interaction experiments identified that PP4 likely promotes Notch signaling within the nucleus of the Notch-receiving cell. Although the PP4 complex is implicated in various cellular processes, its role in the regulation of Wg and Notch pathways was previously uncharacterized. Our study identifies a novel role of PP4 in regulating Notch pathway, resulting in aberrations in Notch-mediated transcriptional regulation of the Wingless ligand. Furthermore, we show that PP4 regulates proliferation independent of its interaction with Notch. |
format | Online Article Text |
id | pubmed-5576076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-55760762017-09-11 The protein phosphatase 4 complex promotes the Notch pathway and wingless transcription Hall, Eric T. Pradhan-Sundd, Tirthadipa Samnani, Faaria Verheyen, Esther M. Biol Open Research Article The Wnt/Wingless (Wg) pathway controls cell fate specification, tissue differentiation and organ development across organisms. Using an in vivo RNAi screen to identify novel kinase and phosphatase regulators of the Wg pathway, we identified subunits of the serine threonine phosphatase Protein Phosphatase 4 (PP4). Knockdown of the catalytic and regulatory subunits of PP4 cause reductions in the Wg pathway targets Senseless and Distal-less. We find that PP4 regulates the Wg pathway by controlling Notch-driven wg transcription. Genetic interaction experiments identified that PP4 likely promotes Notch signaling within the nucleus of the Notch-receiving cell. Although the PP4 complex is implicated in various cellular processes, its role in the regulation of Wg and Notch pathways was previously uncharacterized. Our study identifies a novel role of PP4 in regulating Notch pathway, resulting in aberrations in Notch-mediated transcriptional regulation of the Wingless ligand. Furthermore, we show that PP4 regulates proliferation independent of its interaction with Notch. The Company of Biologists Ltd 2017-06-26 /pmc/articles/PMC5576076/ /pubmed/28652317 http://dx.doi.org/10.1242/bio.025221 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Hall, Eric T. Pradhan-Sundd, Tirthadipa Samnani, Faaria Verheyen, Esther M. The protein phosphatase 4 complex promotes the Notch pathway and wingless transcription |
title | The protein phosphatase 4 complex promotes the Notch pathway and wingless transcription |
title_full | The protein phosphatase 4 complex promotes the Notch pathway and wingless transcription |
title_fullStr | The protein phosphatase 4 complex promotes the Notch pathway and wingless transcription |
title_full_unstemmed | The protein phosphatase 4 complex promotes the Notch pathway and wingless transcription |
title_short | The protein phosphatase 4 complex promotes the Notch pathway and wingless transcription |
title_sort | protein phosphatase 4 complex promotes the notch pathway and wingless transcription |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576076/ https://www.ncbi.nlm.nih.gov/pubmed/28652317 http://dx.doi.org/10.1242/bio.025221 |
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