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Protected sampling is preferable in bronchoscopic studies of the airway microbiome
The aim was to evaluate susceptibility of oropharyngeal contamination with various bronchoscopic sampling techniques. 67 patients with obstructive lung disease and 58 control subjects underwent bronchoscopy with small-volume lavage (SVL) through the working channel, protected bronchoalveolar lavage...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Respiratory Society
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576223/ https://www.ncbi.nlm.nih.gov/pubmed/28875147 http://dx.doi.org/10.1183/23120541.00019-2017 |
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author | Grønseth, Rune Drengenes, Christine Wiker, Harald G. Tangedal, Solveig Xue, Yaxin Husebø, Gunnar Reksten Svanes, Øistein Lehmann, Sverre Aardal, Marit Hoang, Tuyen Kalananthan, Tharmini Hjellestad Martinsen, Einar Marius Orvedal Leiten, Elise Aanerud, Marianne Nordeide, Eli Haaland, Ingvild Jonassen, Inge Bakke, Per Eagan, Tomas |
author_facet | Grønseth, Rune Drengenes, Christine Wiker, Harald G. Tangedal, Solveig Xue, Yaxin Husebø, Gunnar Reksten Svanes, Øistein Lehmann, Sverre Aardal, Marit Hoang, Tuyen Kalananthan, Tharmini Hjellestad Martinsen, Einar Marius Orvedal Leiten, Elise Aanerud, Marianne Nordeide, Eli Haaland, Ingvild Jonassen, Inge Bakke, Per Eagan, Tomas |
author_sort | Grønseth, Rune |
collection | PubMed |
description | The aim was to evaluate susceptibility of oropharyngeal contamination with various bronchoscopic sampling techniques. 67 patients with obstructive lung disease and 58 control subjects underwent bronchoscopy with small-volume lavage (SVL) through the working channel, protected bronchoalveolar lavage (PBAL) and bilateral protected specimen brush (PSB) sampling. Subjects also provided an oral wash (OW) sample, and negative control samples were gathered for each bronchoscopy procedure. DNA encoding bacterial 16S ribosomal RNA was sequenced and bioinformatically processed to cluster into operational taxonomic units (OTU), assign taxonomy and obtain measures of diversity. The proportion of Proteobacteria increased, whereas Firmicutes diminished in the order OW, SVL, PBAL, PSB (p<0.01). The alpha-diversity decreased in the same order (p<0.01). Also, beta-diversity varied by sampling method (p<0.01), and visualisation of principal coordinates analyses indicated that differences in diversity were smaller between OW and SVL and OW and PBAL samples than for OW and the PSB samples. The order of sampling (left versus right first) did not influence alpha- or beta-diversity for PSB samples. Studies of the airway microbiota need to address the potential for oropharyngeal contamination, and protected sampling might represent an acceptable measure to minimise this problem. |
format | Online Article Text |
id | pubmed-5576223 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | European Respiratory Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-55762232017-09-05 Protected sampling is preferable in bronchoscopic studies of the airway microbiome Grønseth, Rune Drengenes, Christine Wiker, Harald G. Tangedal, Solveig Xue, Yaxin Husebø, Gunnar Reksten Svanes, Øistein Lehmann, Sverre Aardal, Marit Hoang, Tuyen Kalananthan, Tharmini Hjellestad Martinsen, Einar Marius Orvedal Leiten, Elise Aanerud, Marianne Nordeide, Eli Haaland, Ingvild Jonassen, Inge Bakke, Per Eagan, Tomas ERJ Open Res Original Articles The aim was to evaluate susceptibility of oropharyngeal contamination with various bronchoscopic sampling techniques. 67 patients with obstructive lung disease and 58 control subjects underwent bronchoscopy with small-volume lavage (SVL) through the working channel, protected bronchoalveolar lavage (PBAL) and bilateral protected specimen brush (PSB) sampling. Subjects also provided an oral wash (OW) sample, and negative control samples were gathered for each bronchoscopy procedure. DNA encoding bacterial 16S ribosomal RNA was sequenced and bioinformatically processed to cluster into operational taxonomic units (OTU), assign taxonomy and obtain measures of diversity. The proportion of Proteobacteria increased, whereas Firmicutes diminished in the order OW, SVL, PBAL, PSB (p<0.01). The alpha-diversity decreased in the same order (p<0.01). Also, beta-diversity varied by sampling method (p<0.01), and visualisation of principal coordinates analyses indicated that differences in diversity were smaller between OW and SVL and OW and PBAL samples than for OW and the PSB samples. The order of sampling (left versus right first) did not influence alpha- or beta-diversity for PSB samples. Studies of the airway microbiota need to address the potential for oropharyngeal contamination, and protected sampling might represent an acceptable measure to minimise this problem. European Respiratory Society 2017-08-30 /pmc/articles/PMC5576223/ /pubmed/28875147 http://dx.doi.org/10.1183/23120541.00019-2017 Text en Copyright ©ERS 2017 http://creativecommons.org/licenses/by-nc/4.0/ This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. |
spellingShingle | Original Articles Grønseth, Rune Drengenes, Christine Wiker, Harald G. Tangedal, Solveig Xue, Yaxin Husebø, Gunnar Reksten Svanes, Øistein Lehmann, Sverre Aardal, Marit Hoang, Tuyen Kalananthan, Tharmini Hjellestad Martinsen, Einar Marius Orvedal Leiten, Elise Aanerud, Marianne Nordeide, Eli Haaland, Ingvild Jonassen, Inge Bakke, Per Eagan, Tomas Protected sampling is preferable in bronchoscopic studies of the airway microbiome |
title | Protected sampling is preferable in bronchoscopic studies of the airway microbiome |
title_full | Protected sampling is preferable in bronchoscopic studies of the airway microbiome |
title_fullStr | Protected sampling is preferable in bronchoscopic studies of the airway microbiome |
title_full_unstemmed | Protected sampling is preferable in bronchoscopic studies of the airway microbiome |
title_short | Protected sampling is preferable in bronchoscopic studies of the airway microbiome |
title_sort | protected sampling is preferable in bronchoscopic studies of the airway microbiome |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576223/ https://www.ncbi.nlm.nih.gov/pubmed/28875147 http://dx.doi.org/10.1183/23120541.00019-2017 |
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