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LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring

The anaphase spindle determines the position of the cytokinesis furrow, such that the contractile ring assembles in an equatorial zone between the two spindle poles. Contractile ring formation is mediated by RhoA activation at the equator by the centralspindlin complex and midzone microtubules. Astr...

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Autores principales: Price, Kari L., Rose, Lesilee S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576900/
https://www.ncbi.nlm.nih.gov/pubmed/28701343
http://dx.doi.org/10.1091/mbc.E16-12-0874
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author Price, Kari L.
Rose, Lesilee S.
author_facet Price, Kari L.
Rose, Lesilee S.
author_sort Price, Kari L.
collection PubMed
description The anaphase spindle determines the position of the cytokinesis furrow, such that the contractile ring assembles in an equatorial zone between the two spindle poles. Contractile ring formation is mediated by RhoA activation at the equator by the centralspindlin complex and midzone microtubules. Astral microtubules also inhibit RhoA accumulation at the poles. In the Caenorhabditis elegans one-cell embryo, the astral microtubule–dependent pathway requires anillin, NOP-1, and LET-99. LET-99 is well characterized for generating the asymmetric cortical localization of the Gα-dependent force-generating complex that positions the spindle during asymmetric division. However, whether the role of LET-99 in cytokinesis is specific to asymmetric division and whether it acts through Gα to promote furrowing are unclear. Here we show that LET-99 contributes to furrowing in both asymmetrically and symmetrically dividing cells, independent of its function in spindle positioning and Gα regulation. LET-99 acts in a pathway parallel to anillin and is required for myosin enrichment into the contractile ring. These and other results suggest a positive feedback model in which LET-99 localizes to the presumptive cleavage furrow in response to the spindle and myosin. Once positioned there, LET-99 enhances myosin accumulation to promote furrowing in both symmetrically and asymmetrically dividing cells.
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spelling pubmed-55769002017-11-16 LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring Price, Kari L. Rose, Lesilee S. Mol Biol Cell Articles The anaphase spindle determines the position of the cytokinesis furrow, such that the contractile ring assembles in an equatorial zone between the two spindle poles. Contractile ring formation is mediated by RhoA activation at the equator by the centralspindlin complex and midzone microtubules. Astral microtubules also inhibit RhoA accumulation at the poles. In the Caenorhabditis elegans one-cell embryo, the astral microtubule–dependent pathway requires anillin, NOP-1, and LET-99. LET-99 is well characterized for generating the asymmetric cortical localization of the Gα-dependent force-generating complex that positions the spindle during asymmetric division. However, whether the role of LET-99 in cytokinesis is specific to asymmetric division and whether it acts through Gα to promote furrowing are unclear. Here we show that LET-99 contributes to furrowing in both asymmetrically and symmetrically dividing cells, independent of its function in spindle positioning and Gα regulation. LET-99 acts in a pathway parallel to anillin and is required for myosin enrichment into the contractile ring. These and other results suggest a positive feedback model in which LET-99 localizes to the presumptive cleavage furrow in response to the spindle and myosin. Once positioned there, LET-99 enhances myosin accumulation to promote furrowing in both symmetrically and asymmetrically dividing cells. The American Society for Cell Biology 2017-09-01 /pmc/articles/PMC5576900/ /pubmed/28701343 http://dx.doi.org/10.1091/mbc.E16-12-0874 Text en © 2017 Price and Rose. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Price, Kari L.
Rose, Lesilee S.
LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring
title LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring
title_full LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring
title_fullStr LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring
title_full_unstemmed LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring
title_short LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring
title_sort let-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576900/
https://www.ncbi.nlm.nih.gov/pubmed/28701343
http://dx.doi.org/10.1091/mbc.E16-12-0874
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