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A casein kinase 1 prevents expulsion of the oocyte meiotic spindle into a polar body by regulating cortical contractility

During female meiosis, haploid eggs are generated from diploid oocytes. This reduction in chromosome number occurs through two highly asymmetric cell divisions, resulting in one large egg and two small polar bodies. Unlike mitosis, where an actomyosin contractile ring forms between the sets of segre...

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Autores principales: Flynn, Jonathan R., McNally, Francis J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576904/
https://www.ncbi.nlm.nih.gov/pubmed/28701347
http://dx.doi.org/10.1091/mbc.E17-01-0056
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author Flynn, Jonathan R.
McNally, Francis J.
author_facet Flynn, Jonathan R.
McNally, Francis J.
author_sort Flynn, Jonathan R.
collection PubMed
description During female meiosis, haploid eggs are generated from diploid oocytes. This reduction in chromosome number occurs through two highly asymmetric cell divisions, resulting in one large egg and two small polar bodies. Unlike mitosis, where an actomyosin contractile ring forms between the sets of segregating chromosomes, the meiotic contractile ring forms on the cortex adjacent to one spindle pole, then ingresses down the length of the spindle to position itself at the exact midpoint between the two sets of segregating chromosomes. Depletion of casein kinase 1 gamma (CSNK-1) in Caenorhabditis elegans led to the formation of large polar bodies that contain all maternal DNA, because the contractile ring ingressed past the spindle midpoint. Depletion of CSNK-1 also resulted in the formation of deep membrane invaginations during meiosis, suggesting an effect on cortical myosin. Both myosin and anillin assemble into dynamic rho-dependent cortical patches that rapidly disassemble in wild-type embryos. CSNK-1 was required for disassembly of both myosin patches and anillin patches. Disassembly of anillin patches was myosin independent, suggesting that CSNK-1 prevents expulsion of the entire meiotic spindle into a polar body by negatively regulating the rho pathway rather than through direct inhibition of myosin.
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spelling pubmed-55769042017-11-16 A casein kinase 1 prevents expulsion of the oocyte meiotic spindle into a polar body by regulating cortical contractility Flynn, Jonathan R. McNally, Francis J. Mol Biol Cell Articles During female meiosis, haploid eggs are generated from diploid oocytes. This reduction in chromosome number occurs through two highly asymmetric cell divisions, resulting in one large egg and two small polar bodies. Unlike mitosis, where an actomyosin contractile ring forms between the sets of segregating chromosomes, the meiotic contractile ring forms on the cortex adjacent to one spindle pole, then ingresses down the length of the spindle to position itself at the exact midpoint between the two sets of segregating chromosomes. Depletion of casein kinase 1 gamma (CSNK-1) in Caenorhabditis elegans led to the formation of large polar bodies that contain all maternal DNA, because the contractile ring ingressed past the spindle midpoint. Depletion of CSNK-1 also resulted in the formation of deep membrane invaginations during meiosis, suggesting an effect on cortical myosin. Both myosin and anillin assemble into dynamic rho-dependent cortical patches that rapidly disassemble in wild-type embryos. CSNK-1 was required for disassembly of both myosin patches and anillin patches. Disassembly of anillin patches was myosin independent, suggesting that CSNK-1 prevents expulsion of the entire meiotic spindle into a polar body by negatively regulating the rho pathway rather than through direct inhibition of myosin. The American Society for Cell Biology 2017-09-01 /pmc/articles/PMC5576904/ /pubmed/28701347 http://dx.doi.org/10.1091/mbc.E17-01-0056 Text en © 2017 Flynn and McNally. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Flynn, Jonathan R.
McNally, Francis J.
A casein kinase 1 prevents expulsion of the oocyte meiotic spindle into a polar body by regulating cortical contractility
title A casein kinase 1 prevents expulsion of the oocyte meiotic spindle into a polar body by regulating cortical contractility
title_full A casein kinase 1 prevents expulsion of the oocyte meiotic spindle into a polar body by regulating cortical contractility
title_fullStr A casein kinase 1 prevents expulsion of the oocyte meiotic spindle into a polar body by regulating cortical contractility
title_full_unstemmed A casein kinase 1 prevents expulsion of the oocyte meiotic spindle into a polar body by regulating cortical contractility
title_short A casein kinase 1 prevents expulsion of the oocyte meiotic spindle into a polar body by regulating cortical contractility
title_sort casein kinase 1 prevents expulsion of the oocyte meiotic spindle into a polar body by regulating cortical contractility
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576904/
https://www.ncbi.nlm.nih.gov/pubmed/28701347
http://dx.doi.org/10.1091/mbc.E17-01-0056
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