Cargando…
Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae
Rapid detection of carbapenemase-producing Enterobacteriaceae (CPE) represents a major challenge for microbiology laboratories. We evaluated the BYG Carba v2.0 using a simplified protocol, which detects CPE in less than 30 minutes. This new procedure reduces the hands-on-time from 5 to one minute an...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577128/ https://www.ncbi.nlm.nih.gov/pubmed/28855689 http://dx.doi.org/10.1038/s41598-017-09820-y |
_version_ | 1783260290650996736 |
---|---|
author | Bogaerts, Pierre Oueslati, Saoussen Meunier, Danièle Nonhoff, Claire Yunus, Sami Massart, Marion Denis, Olivier Woodford, Neil Hopkins, Katie L. Naas, Thierry Dortet, Laurent Huang, Te-Din Glupczynski, Youri |
author_facet | Bogaerts, Pierre Oueslati, Saoussen Meunier, Danièle Nonhoff, Claire Yunus, Sami Massart, Marion Denis, Olivier Woodford, Neil Hopkins, Katie L. Naas, Thierry Dortet, Laurent Huang, Te-Din Glupczynski, Youri |
author_sort | Bogaerts, Pierre |
collection | PubMed |
description | Rapid detection of carbapenemase-producing Enterobacteriaceae (CPE) represents a major challenge for microbiology laboratories. We evaluated the BYG Carba v2.0 using a simplified protocol, which detects CPE in less than 30 minutes. This new procedure reduces the hands-on-time from 5 to one minute and only requires a limited amount of material (one to three colonies) thereby preventing the need for subculturing bacterial isolates to reach a larger amount of pure biomass. This multicentre study involved four European reference laboratories. For the 1181 isolates tested across four centres, BYG Carba v2.0 yielded overall sensitivity and specificity of 96.3% (CI95: 94.5–97.5) and 99.7% (CI95: 98.6–100) respectively. Considering only the 670 consecutive isolates tested prospectively, the BYG Carba v2.0 displayed overall positive and negative predictive values of 99.7% (CI95: 95.4–98.9) and 97.5% (CI95: 94.9–98.8). Regarding time to positivity, 85% of CPE detected were positive within ten minutes. The BYG Carba v2.0 is a new highly simplified, rapid and accurate electrochemical assay discriminating between CPE and non-CPE in less than 30 min. The real-time quantified signal allows objective and traceable interpretation of the results. |
format | Online Article Text |
id | pubmed-5577128 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55771282017-09-01 Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae Bogaerts, Pierre Oueslati, Saoussen Meunier, Danièle Nonhoff, Claire Yunus, Sami Massart, Marion Denis, Olivier Woodford, Neil Hopkins, Katie L. Naas, Thierry Dortet, Laurent Huang, Te-Din Glupczynski, Youri Sci Rep Article Rapid detection of carbapenemase-producing Enterobacteriaceae (CPE) represents a major challenge for microbiology laboratories. We evaluated the BYG Carba v2.0 using a simplified protocol, which detects CPE in less than 30 minutes. This new procedure reduces the hands-on-time from 5 to one minute and only requires a limited amount of material (one to three colonies) thereby preventing the need for subculturing bacterial isolates to reach a larger amount of pure biomass. This multicentre study involved four European reference laboratories. For the 1181 isolates tested across four centres, BYG Carba v2.0 yielded overall sensitivity and specificity of 96.3% (CI95: 94.5–97.5) and 99.7% (CI95: 98.6–100) respectively. Considering only the 670 consecutive isolates tested prospectively, the BYG Carba v2.0 displayed overall positive and negative predictive values of 99.7% (CI95: 95.4–98.9) and 97.5% (CI95: 94.9–98.8). Regarding time to positivity, 85% of CPE detected were positive within ten minutes. The BYG Carba v2.0 is a new highly simplified, rapid and accurate electrochemical assay discriminating between CPE and non-CPE in less than 30 min. The real-time quantified signal allows objective and traceable interpretation of the results. Nature Publishing Group UK 2017-08-30 /pmc/articles/PMC5577128/ /pubmed/28855689 http://dx.doi.org/10.1038/s41598-017-09820-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Bogaerts, Pierre Oueslati, Saoussen Meunier, Danièle Nonhoff, Claire Yunus, Sami Massart, Marion Denis, Olivier Woodford, Neil Hopkins, Katie L. Naas, Thierry Dortet, Laurent Huang, Te-Din Glupczynski, Youri Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae |
title | Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae |
title_full | Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae |
title_fullStr | Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae |
title_full_unstemmed | Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae |
title_short | Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae |
title_sort | multicentre evaluation of the byg carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing enterobacteriaceae |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577128/ https://www.ncbi.nlm.nih.gov/pubmed/28855689 http://dx.doi.org/10.1038/s41598-017-09820-y |
work_keys_str_mv | AT bogaertspierre multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT oueslatisaoussen multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT meunierdaniele multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT nonhoffclaire multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT yunussami multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT massartmarion multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT denisolivier multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT woodfordneil multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT hopkinskatiel multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT naasthierry multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT dortetlaurent multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT huangtedin multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae AT glupczynskiyouri multicentreevaluationofthebygcarbav20testasimplifiedelectrochemicalassayfortherapidlaboratorydetectionofcarbapenemaseproducingenterobacteriaceae |