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Immunization with truncated envelope protein of Zika virus induces protective immune response in mice

The global spread of Zika virus (ZIKV) as well as its unexpected link to infant microcephaly have resulted in serious public health concerns. No antiviral drugs against ZIKV is currently available, and vaccine development is of high priority to prepare for potential ZIKV pandemic. In the present stu...

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Detalles Bibliográficos
Autores principales: Han, Jian-Feng, Qiu, Yang, Yu, Jiu-Yang, Wang, Hong-Jiang, Deng, Yong-Qiang, Li, Xiao-Feng, Zhao, Hui, Sun, Han-Xiao, Qin, Cheng-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577204/
https://www.ncbi.nlm.nih.gov/pubmed/28855646
http://dx.doi.org/10.1038/s41598-017-10595-5
Descripción
Sumario:The global spread of Zika virus (ZIKV) as well as its unexpected link to infant microcephaly have resulted in serious public health concerns. No antiviral drugs against ZIKV is currently available, and vaccine development is of high priority to prepare for potential ZIKV pandemic. In the present study, a truncated E protein with the N-terminal 90% region reserved (E90) from a contemporary ZIKV strain was cloned and expressed in Escherichia coli, purified by a Ni-NTA column, and characterized by Western blotting assays. Immunization with recombinant E90 induced robust ZIKV-specific humoral response in adult BALB/c mice. Passive transfer of the antisera from E90-immunized mice conferred full protection against lethal ZIKV challenge in a neonatal mice model. Our results indicate that recombinant ZIKV E90 described here represents as a promising ZIKV subunit vaccine that deserves further clinical development.