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Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress
As a nongenetic engineering technique, adaptive evolution is an effective and easy-to-operate approach to strain improvement. In this work, a commercial Thermoanaerobacterium aotearoense SCUT27/Δldh-G58 was successfully isolated via sequential batch fermentation with step-increased carbon concentrat...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577258/ https://www.ncbi.nlm.nih.gov/pubmed/28855699 http://dx.doi.org/10.1038/s41598-017-10514-8 |
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author | Zhu, Muzi Fan, Wudi Cha, Yaping Yang, Xiaofeng Lai, Zhicheng Li, Shuang Wang, Xiaoning |
author_facet | Zhu, Muzi Fan, Wudi Cha, Yaping Yang, Xiaofeng Lai, Zhicheng Li, Shuang Wang, Xiaoning |
author_sort | Zhu, Muzi |
collection | PubMed |
description | As a nongenetic engineering technique, adaptive evolution is an effective and easy-to-operate approach to strain improvement. In this work, a commercial Thermoanaerobacterium aotearoense SCUT27/Δldh-G58 was successfully isolated via sequential batch fermentation with step-increased carbon concentrations. Mutants were isolated under selective high osmotic pressures for 58 passages. The evolved isolate rapidly catabolized sugars at high concentrations and subsequently produced ethanol with good yield. A 1.6-fold improvement of ethanol production was achieved in a medium containing 120 g/L of carbon substrate using the evolved strain, compared to the start strain. The analysis of transcriptome and intracellular solute pools suggested that the adaptive evolution altered the synthesis of some compatible solutes and activated the DNA repair system in the two Thermoanaerobacterium sp. evolved strains. Overall, the results indicated the potential of adaptive evolution as a simple and effective tool for the modification and optimization of industrial microorganisms. |
format | Online Article Text |
id | pubmed-5577258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55772582017-09-01 Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress Zhu, Muzi Fan, Wudi Cha, Yaping Yang, Xiaofeng Lai, Zhicheng Li, Shuang Wang, Xiaoning Sci Rep Article As a nongenetic engineering technique, adaptive evolution is an effective and easy-to-operate approach to strain improvement. In this work, a commercial Thermoanaerobacterium aotearoense SCUT27/Δldh-G58 was successfully isolated via sequential batch fermentation with step-increased carbon concentrations. Mutants were isolated under selective high osmotic pressures for 58 passages. The evolved isolate rapidly catabolized sugars at high concentrations and subsequently produced ethanol with good yield. A 1.6-fold improvement of ethanol production was achieved in a medium containing 120 g/L of carbon substrate using the evolved strain, compared to the start strain. The analysis of transcriptome and intracellular solute pools suggested that the adaptive evolution altered the synthesis of some compatible solutes and activated the DNA repair system in the two Thermoanaerobacterium sp. evolved strains. Overall, the results indicated the potential of adaptive evolution as a simple and effective tool for the modification and optimization of industrial microorganisms. Nature Publishing Group UK 2017-08-30 /pmc/articles/PMC5577258/ /pubmed/28855699 http://dx.doi.org/10.1038/s41598-017-10514-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zhu, Muzi Fan, Wudi Cha, Yaping Yang, Xiaofeng Lai, Zhicheng Li, Shuang Wang, Xiaoning Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress |
title | Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress |
title_full | Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress |
title_fullStr | Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress |
title_full_unstemmed | Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress |
title_short | Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress |
title_sort | dynamic cell responses in thermoanaerobacterium sp. under hyperosmotic stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577258/ https://www.ncbi.nlm.nih.gov/pubmed/28855699 http://dx.doi.org/10.1038/s41598-017-10514-8 |
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