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Prevalence and Genetic Characteristics of Staphylococcus aureus and Staphylococcus argenteus Isolates Harboring Panton-Valentine Leukocidin, Enterotoxins, and TSST-1 Genes from Food Handlers in Myanmar

Asymptomatic carriers of toxigenic Staphylococcus aureus are potential source of diseases, including food poisoning. Toxigenic potential and genetic traits of colonizing S. aureus were investigated for 563 healthy food handlers in Myanmar. Carriage of S. aureus was found in 110 individuals (19.5%),...

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Autores principales: Aung, Meiji Soe, San, Thida, Aye, Mya Mya, Mya, San, Maw, Win Win, Zan, Khin Nyein, Htut, Wut Hmone Win, Kawaguchiya, Mitsuyo, Urushibara, Noriko, Kobayashi, Nobumichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577575/
https://www.ncbi.nlm.nih.gov/pubmed/28777321
http://dx.doi.org/10.3390/toxins9080241
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author Aung, Meiji Soe
San, Thida
Aye, Mya Mya
Mya, San
Maw, Win Win
Zan, Khin Nyein
Htut, Wut Hmone Win
Kawaguchiya, Mitsuyo
Urushibara, Noriko
Kobayashi, Nobumichi
author_facet Aung, Meiji Soe
San, Thida
Aye, Mya Mya
Mya, San
Maw, Win Win
Zan, Khin Nyein
Htut, Wut Hmone Win
Kawaguchiya, Mitsuyo
Urushibara, Noriko
Kobayashi, Nobumichi
author_sort Aung, Meiji Soe
collection PubMed
description Asymptomatic carriers of toxigenic Staphylococcus aureus are potential source of diseases, including food poisoning. Toxigenic potential and genetic traits of colonizing S. aureus were investigated for 563 healthy food handlers in Myanmar. Carriage of S. aureus was found in 110 individuals (19.5%), and a total of 144 S. aureus isolates were recovered from nasal cavities (110 isolates) and hands (34 isolates). Panton-Valentine leucocidin genes (pvl) were detected in 18 isolates (12.5%), among which 11 isolates were classified into coa-VIa, agr type III, and ST1930 (CC96) that had been also detected in pvl-positive clinical isolates in Myanmar. A pvl-positive, ST2250 nasal isolate was identified as S. argenteus, a novel coagulase-positive staphylococcus species. Toxic shock syndrome toxin-1 (TSST-1) gene was detected in five pvl-negative isolates. All of the 144 isolates harbored at least one of the 21 enterotoxin(-like) gene(s). The most prevalent enterotoxin(-like) gene was selw (98%), followed by selx (97%), sei (28%), sely (28%), sem (26%), sel (24%), and sea and sec (22% each). Considerable genetic diversity with five groups was detected for selw. The present study revealed the relatively high rate of pvl, as well as the wide distribution of enterotoxin(-like) genes among colonizing S. aureus in Myanmar.
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spelling pubmed-55775752017-09-05 Prevalence and Genetic Characteristics of Staphylococcus aureus and Staphylococcus argenteus Isolates Harboring Panton-Valentine Leukocidin, Enterotoxins, and TSST-1 Genes from Food Handlers in Myanmar Aung, Meiji Soe San, Thida Aye, Mya Mya Mya, San Maw, Win Win Zan, Khin Nyein Htut, Wut Hmone Win Kawaguchiya, Mitsuyo Urushibara, Noriko Kobayashi, Nobumichi Toxins (Basel) Article Asymptomatic carriers of toxigenic Staphylococcus aureus are potential source of diseases, including food poisoning. Toxigenic potential and genetic traits of colonizing S. aureus were investigated for 563 healthy food handlers in Myanmar. Carriage of S. aureus was found in 110 individuals (19.5%), and a total of 144 S. aureus isolates were recovered from nasal cavities (110 isolates) and hands (34 isolates). Panton-Valentine leucocidin genes (pvl) were detected in 18 isolates (12.5%), among which 11 isolates were classified into coa-VIa, agr type III, and ST1930 (CC96) that had been also detected in pvl-positive clinical isolates in Myanmar. A pvl-positive, ST2250 nasal isolate was identified as S. argenteus, a novel coagulase-positive staphylococcus species. Toxic shock syndrome toxin-1 (TSST-1) gene was detected in five pvl-negative isolates. All of the 144 isolates harbored at least one of the 21 enterotoxin(-like) gene(s). The most prevalent enterotoxin(-like) gene was selw (98%), followed by selx (97%), sei (28%), sely (28%), sem (26%), sel (24%), and sea and sec (22% each). Considerable genetic diversity with five groups was detected for selw. The present study revealed the relatively high rate of pvl, as well as the wide distribution of enterotoxin(-like) genes among colonizing S. aureus in Myanmar. MDPI 2017-08-04 /pmc/articles/PMC5577575/ /pubmed/28777321 http://dx.doi.org/10.3390/toxins9080241 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aung, Meiji Soe
San, Thida
Aye, Mya Mya
Mya, San
Maw, Win Win
Zan, Khin Nyein
Htut, Wut Hmone Win
Kawaguchiya, Mitsuyo
Urushibara, Noriko
Kobayashi, Nobumichi
Prevalence and Genetic Characteristics of Staphylococcus aureus and Staphylococcus argenteus Isolates Harboring Panton-Valentine Leukocidin, Enterotoxins, and TSST-1 Genes from Food Handlers in Myanmar
title Prevalence and Genetic Characteristics of Staphylococcus aureus and Staphylococcus argenteus Isolates Harboring Panton-Valentine Leukocidin, Enterotoxins, and TSST-1 Genes from Food Handlers in Myanmar
title_full Prevalence and Genetic Characteristics of Staphylococcus aureus and Staphylococcus argenteus Isolates Harboring Panton-Valentine Leukocidin, Enterotoxins, and TSST-1 Genes from Food Handlers in Myanmar
title_fullStr Prevalence and Genetic Characteristics of Staphylococcus aureus and Staphylococcus argenteus Isolates Harboring Panton-Valentine Leukocidin, Enterotoxins, and TSST-1 Genes from Food Handlers in Myanmar
title_full_unstemmed Prevalence and Genetic Characteristics of Staphylococcus aureus and Staphylococcus argenteus Isolates Harboring Panton-Valentine Leukocidin, Enterotoxins, and TSST-1 Genes from Food Handlers in Myanmar
title_short Prevalence and Genetic Characteristics of Staphylococcus aureus and Staphylococcus argenteus Isolates Harboring Panton-Valentine Leukocidin, Enterotoxins, and TSST-1 Genes from Food Handlers in Myanmar
title_sort prevalence and genetic characteristics of staphylococcus aureus and staphylococcus argenteus isolates harboring panton-valentine leukocidin, enterotoxins, and tsst-1 genes from food handlers in myanmar
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577575/
https://www.ncbi.nlm.nih.gov/pubmed/28777321
http://dx.doi.org/10.3390/toxins9080241
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