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A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria

A marine-derived Stilbella fimetaria fungal strain was screened for new bioactive compounds based on two different approaches: (i) bio-guided approach using cytotoxicity and antimicrobial bioassays; and (ii) dereplication based approach using liquid chromatography with both diode array detection and...

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Autores principales: Kildgaard, Sara, Subko, Karolina, Phillips, Emma, Goidts, Violaine, de la Cruz, Mercedes, Díaz, Caridad, Gotfredsen, Charlotte H., Andersen, Birgitte, Frisvad, Jens C., Nielsen, Kristian F., Larsen, Thomas O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577607/
https://www.ncbi.nlm.nih.gov/pubmed/28805711
http://dx.doi.org/10.3390/md15080253
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author Kildgaard, Sara
Subko, Karolina
Phillips, Emma
Goidts, Violaine
de la Cruz, Mercedes
Díaz, Caridad
Gotfredsen, Charlotte H.
Andersen, Birgitte
Frisvad, Jens C.
Nielsen, Kristian F.
Larsen, Thomas O.
author_facet Kildgaard, Sara
Subko, Karolina
Phillips, Emma
Goidts, Violaine
de la Cruz, Mercedes
Díaz, Caridad
Gotfredsen, Charlotte H.
Andersen, Birgitte
Frisvad, Jens C.
Nielsen, Kristian F.
Larsen, Thomas O.
author_sort Kildgaard, Sara
collection PubMed
description A marine-derived Stilbella fimetaria fungal strain was screened for new bioactive compounds based on two different approaches: (i) bio-guided approach using cytotoxicity and antimicrobial bioassays; and (ii) dereplication based approach using liquid chromatography with both diode array detection and high resolution mass spectrometry. This led to the discovery of several bioactive compound families with different biosynthetic origins, including pimarane-type diterpenoids and hybrid polyketide-non ribosomal peptide derived compounds. Prefractionation before bioassay screening proved to be a great aid in the dereplication process, since separate fractions displaying different bioactivities allowed a quick tentative identification of known antimicrobial compounds and of potential new analogues. A new pimarane-type diterpene, myrocin F, was discovered in trace amounts and displayed cytotoxicity towards various cancer cell lines. Further media optimization led to increased production followed by the purification and bioactivity screening of several new and known pimarane-type diterpenoids. A known broad-spectrum antifungal compound, ilicicolin H, was purified along with two new analogues, hydroxyl-ilicicolin H and ilicicolin I, and their antifungal activity was evaluated.
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spelling pubmed-55776072017-09-05 A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria Kildgaard, Sara Subko, Karolina Phillips, Emma Goidts, Violaine de la Cruz, Mercedes Díaz, Caridad Gotfredsen, Charlotte H. Andersen, Birgitte Frisvad, Jens C. Nielsen, Kristian F. Larsen, Thomas O. Mar Drugs Article A marine-derived Stilbella fimetaria fungal strain was screened for new bioactive compounds based on two different approaches: (i) bio-guided approach using cytotoxicity and antimicrobial bioassays; and (ii) dereplication based approach using liquid chromatography with both diode array detection and high resolution mass spectrometry. This led to the discovery of several bioactive compound families with different biosynthetic origins, including pimarane-type diterpenoids and hybrid polyketide-non ribosomal peptide derived compounds. Prefractionation before bioassay screening proved to be a great aid in the dereplication process, since separate fractions displaying different bioactivities allowed a quick tentative identification of known antimicrobial compounds and of potential new analogues. A new pimarane-type diterpene, myrocin F, was discovered in trace amounts and displayed cytotoxicity towards various cancer cell lines. Further media optimization led to increased production followed by the purification and bioactivity screening of several new and known pimarane-type diterpenoids. A known broad-spectrum antifungal compound, ilicicolin H, was purified along with two new analogues, hydroxyl-ilicicolin H and ilicicolin I, and their antifungal activity was evaluated. MDPI 2017-08-13 /pmc/articles/PMC5577607/ /pubmed/28805711 http://dx.doi.org/10.3390/md15080253 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kildgaard, Sara
Subko, Karolina
Phillips, Emma
Goidts, Violaine
de la Cruz, Mercedes
Díaz, Caridad
Gotfredsen, Charlotte H.
Andersen, Birgitte
Frisvad, Jens C.
Nielsen, Kristian F.
Larsen, Thomas O.
A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria
title A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria
title_full A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria
title_fullStr A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria
title_full_unstemmed A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria
title_short A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria
title_sort dereplication and bioguided discovery approach to reveal new compounds from a marine-derived fungus stilbella fimetaria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577607/
https://www.ncbi.nlm.nih.gov/pubmed/28805711
http://dx.doi.org/10.3390/md15080253
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