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Chemical Constituents from Hericium erinaceus Promote Neuronal Survival and Potentiate Neurite Outgrowth via the TrkA/Erk1/2 Pathway

Hericium erinaceus is a culinary-medicinal mushroom used traditionally in Eastern Asia to improve memory. In this work, we investigated the neuroprotective and neuritogenic effects of the secondary metabolites isolated from the MeOH extract of cultured mycelium of H. erinaceus and the primary mechan...

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Autores principales: Zhang, Cheng-Chen, Cao, Chen-Yu, Kubo, Miwa, Harada, Kenichi, Yan, Xi-Tao, Fukuyama, Yoshiyasu, Gao, Jin-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578049/
https://www.ncbi.nlm.nih.gov/pubmed/28758954
http://dx.doi.org/10.3390/ijms18081659
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author Zhang, Cheng-Chen
Cao, Chen-Yu
Kubo, Miwa
Harada, Kenichi
Yan, Xi-Tao
Fukuyama, Yoshiyasu
Gao, Jin-Ming
author_facet Zhang, Cheng-Chen
Cao, Chen-Yu
Kubo, Miwa
Harada, Kenichi
Yan, Xi-Tao
Fukuyama, Yoshiyasu
Gao, Jin-Ming
author_sort Zhang, Cheng-Chen
collection PubMed
description Hericium erinaceus is a culinary-medicinal mushroom used traditionally in Eastern Asia to improve memory. In this work, we investigated the neuroprotective and neuritogenic effects of the secondary metabolites isolated from the MeOH extract of cultured mycelium of H. erinaceus and the primary mechanisms involved. One new dihydropyridine compound (6) and one new natural product (2) together with five known compounds (1,3–5,7) were obtained and their structures were elucidated by spectroscopic analysis, including 2D NMR and HRMS. The cell-based screening for bioactivity showed that 4-chloro-3,5-dimethoxybenzoic methyl ester (1) and a cyathane diterpenoid, erincine A (3), not only potentiated NGF-induced neurite outgrowth but also protected neuronally-differentiated cells against deprivation of NGF in PC12 pheochromocytoma cells. Additionally, compound 3 induced neuritogenesis in primary rat cortex neurons. Furthermore, our results revealed that TrkA-mediated and Erk1/2-dependant pathways could be involved in 1 and 3-promoted NGF-induced neurite outgrowth in PC12 cells.
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spelling pubmed-55780492017-09-05 Chemical Constituents from Hericium erinaceus Promote Neuronal Survival and Potentiate Neurite Outgrowth via the TrkA/Erk1/2 Pathway Zhang, Cheng-Chen Cao, Chen-Yu Kubo, Miwa Harada, Kenichi Yan, Xi-Tao Fukuyama, Yoshiyasu Gao, Jin-Ming Int J Mol Sci Article Hericium erinaceus is a culinary-medicinal mushroom used traditionally in Eastern Asia to improve memory. In this work, we investigated the neuroprotective and neuritogenic effects of the secondary metabolites isolated from the MeOH extract of cultured mycelium of H. erinaceus and the primary mechanisms involved. One new dihydropyridine compound (6) and one new natural product (2) together with five known compounds (1,3–5,7) were obtained and their structures were elucidated by spectroscopic analysis, including 2D NMR and HRMS. The cell-based screening for bioactivity showed that 4-chloro-3,5-dimethoxybenzoic methyl ester (1) and a cyathane diterpenoid, erincine A (3), not only potentiated NGF-induced neurite outgrowth but also protected neuronally-differentiated cells against deprivation of NGF in PC12 pheochromocytoma cells. Additionally, compound 3 induced neuritogenesis in primary rat cortex neurons. Furthermore, our results revealed that TrkA-mediated and Erk1/2-dependant pathways could be involved in 1 and 3-promoted NGF-induced neurite outgrowth in PC12 cells. MDPI 2017-07-30 /pmc/articles/PMC5578049/ /pubmed/28758954 http://dx.doi.org/10.3390/ijms18081659 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Cheng-Chen
Cao, Chen-Yu
Kubo, Miwa
Harada, Kenichi
Yan, Xi-Tao
Fukuyama, Yoshiyasu
Gao, Jin-Ming
Chemical Constituents from Hericium erinaceus Promote Neuronal Survival and Potentiate Neurite Outgrowth via the TrkA/Erk1/2 Pathway
title Chemical Constituents from Hericium erinaceus Promote Neuronal Survival and Potentiate Neurite Outgrowth via the TrkA/Erk1/2 Pathway
title_full Chemical Constituents from Hericium erinaceus Promote Neuronal Survival and Potentiate Neurite Outgrowth via the TrkA/Erk1/2 Pathway
title_fullStr Chemical Constituents from Hericium erinaceus Promote Neuronal Survival and Potentiate Neurite Outgrowth via the TrkA/Erk1/2 Pathway
title_full_unstemmed Chemical Constituents from Hericium erinaceus Promote Neuronal Survival and Potentiate Neurite Outgrowth via the TrkA/Erk1/2 Pathway
title_short Chemical Constituents from Hericium erinaceus Promote Neuronal Survival and Potentiate Neurite Outgrowth via the TrkA/Erk1/2 Pathway
title_sort chemical constituents from hericium erinaceus promote neuronal survival and potentiate neurite outgrowth via the trka/erk1/2 pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578049/
https://www.ncbi.nlm.nih.gov/pubmed/28758954
http://dx.doi.org/10.3390/ijms18081659
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