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Anti-Atherosclerotic Action of Agmatine in ApoE-Knockout Mice

Atherosclerosis is an inflammatory disease in which dysfunction of mitochondria play an important role, and disorders of lipid management intensify this process. Agmatine, an endogenous polyamine formed by decarboxylation of arginine, exerts a protective effect on mitochondria and modulates fatty ac...

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Autores principales: Wiśniewska, Anna, Olszanecki, Rafał, Totoń-Żurańska, Justyna, Kuś, Katarzyna, Stachowicz, Aneta, Suski, Maciej, Gębska, Anna, Gajda, Mariusz, Jawień, Jacek, Korbut, Ryszard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578096/
https://www.ncbi.nlm.nih.gov/pubmed/28777310
http://dx.doi.org/10.3390/ijms18081706
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author Wiśniewska, Anna
Olszanecki, Rafał
Totoń-Żurańska, Justyna
Kuś, Katarzyna
Stachowicz, Aneta
Suski, Maciej
Gębska, Anna
Gajda, Mariusz
Jawień, Jacek
Korbut, Ryszard
author_facet Wiśniewska, Anna
Olszanecki, Rafał
Totoń-Żurańska, Justyna
Kuś, Katarzyna
Stachowicz, Aneta
Suski, Maciej
Gębska, Anna
Gajda, Mariusz
Jawień, Jacek
Korbut, Ryszard
author_sort Wiśniewska, Anna
collection PubMed
description Atherosclerosis is an inflammatory disease in which dysfunction of mitochondria play an important role, and disorders of lipid management intensify this process. Agmatine, an endogenous polyamine formed by decarboxylation of arginine, exerts a protective effect on mitochondria and modulates fatty acid metabolism. We investigated the effect of exogenous agmatine on the development of atherosclerosis and changes in lipid profile in apolipoprotein E knockout (apoE-/-) mice. Agmatine caused an approximate 40% decrease of atherosclerotic lesions, as estimated by en face and cross-section methods with an influence on macrophage but not on smooth muscle content in the plaques. Agmatine treatment did not changed gelatinase activity within the plaque area. What is more, the action of agmatine was associated with an increase in the number of high density lipoproteins (HDL) in blood. Real-Time PCR analysis showed that agmatine modulates liver mRNA levels of many factors involved in oxidation of fatty acid and cholesterol biosynthesis. Two-dimensional electrophoresis coupled with mass spectrometry identified 27 differentially expressed mitochondrial proteins upon agmatine treatment in the liver of apoE-/- mice, mostly proteins related to metabolism and apoptosis. In conclusion, prolonged administration of agmatine inhibits atherosclerosis in apoE-/- mice; however, the exact mechanisms linking observed changes and elevations of HDL plasma require further investigation.
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spelling pubmed-55780962017-09-05 Anti-Atherosclerotic Action of Agmatine in ApoE-Knockout Mice Wiśniewska, Anna Olszanecki, Rafał Totoń-Żurańska, Justyna Kuś, Katarzyna Stachowicz, Aneta Suski, Maciej Gębska, Anna Gajda, Mariusz Jawień, Jacek Korbut, Ryszard Int J Mol Sci Article Atherosclerosis is an inflammatory disease in which dysfunction of mitochondria play an important role, and disorders of lipid management intensify this process. Agmatine, an endogenous polyamine formed by decarboxylation of arginine, exerts a protective effect on mitochondria and modulates fatty acid metabolism. We investigated the effect of exogenous agmatine on the development of atherosclerosis and changes in lipid profile in apolipoprotein E knockout (apoE-/-) mice. Agmatine caused an approximate 40% decrease of atherosclerotic lesions, as estimated by en face and cross-section methods with an influence on macrophage but not on smooth muscle content in the plaques. Agmatine treatment did not changed gelatinase activity within the plaque area. What is more, the action of agmatine was associated with an increase in the number of high density lipoproteins (HDL) in blood. Real-Time PCR analysis showed that agmatine modulates liver mRNA levels of many factors involved in oxidation of fatty acid and cholesterol biosynthesis. Two-dimensional electrophoresis coupled with mass spectrometry identified 27 differentially expressed mitochondrial proteins upon agmatine treatment in the liver of apoE-/- mice, mostly proteins related to metabolism and apoptosis. In conclusion, prolonged administration of agmatine inhibits atherosclerosis in apoE-/- mice; however, the exact mechanisms linking observed changes and elevations of HDL plasma require further investigation. MDPI 2017-08-04 /pmc/articles/PMC5578096/ /pubmed/28777310 http://dx.doi.org/10.3390/ijms18081706 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wiśniewska, Anna
Olszanecki, Rafał
Totoń-Żurańska, Justyna
Kuś, Katarzyna
Stachowicz, Aneta
Suski, Maciej
Gębska, Anna
Gajda, Mariusz
Jawień, Jacek
Korbut, Ryszard
Anti-Atherosclerotic Action of Agmatine in ApoE-Knockout Mice
title Anti-Atherosclerotic Action of Agmatine in ApoE-Knockout Mice
title_full Anti-Atherosclerotic Action of Agmatine in ApoE-Knockout Mice
title_fullStr Anti-Atherosclerotic Action of Agmatine in ApoE-Knockout Mice
title_full_unstemmed Anti-Atherosclerotic Action of Agmatine in ApoE-Knockout Mice
title_short Anti-Atherosclerotic Action of Agmatine in ApoE-Knockout Mice
title_sort anti-atherosclerotic action of agmatine in apoe-knockout mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578096/
https://www.ncbi.nlm.nih.gov/pubmed/28777310
http://dx.doi.org/10.3390/ijms18081706
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