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Endolysosomal Degradation of Allergenic Ole e 1-Like Proteins: Analysis of Proteolytic Cleavage Sites Revealing T Cell Epitope-Containing Peptides

Knowledge of the susceptibility of proteins to endolysosomal proteases provides valuable information on immunogenicity. Though Ole e 1-like proteins are considered relevant allergens, little is known about their immunogenic properties and T cell epitopes. Thus, six representative molecules, i.e., Ol...

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Autores principales: Wildner, Sabrina, Elsässer, Brigitta, Stemeseder, Teresa, Briza, Peter, Soh, Wai Tuck, Villalba, Mayte, Lidholm, Jonas, Brandstetter, Hans, Gadermaier, Gabriele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578169/
https://www.ncbi.nlm.nih.gov/pubmed/28812992
http://dx.doi.org/10.3390/ijms18081780
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author Wildner, Sabrina
Elsässer, Brigitta
Stemeseder, Teresa
Briza, Peter
Soh, Wai Tuck
Villalba, Mayte
Lidholm, Jonas
Brandstetter, Hans
Gadermaier, Gabriele
author_facet Wildner, Sabrina
Elsässer, Brigitta
Stemeseder, Teresa
Briza, Peter
Soh, Wai Tuck
Villalba, Mayte
Lidholm, Jonas
Brandstetter, Hans
Gadermaier, Gabriele
author_sort Wildner, Sabrina
collection PubMed
description Knowledge of the susceptibility of proteins to endolysosomal proteases provides valuable information on immunogenicity. Though Ole e 1-like proteins are considered relevant allergens, little is known about their immunogenic properties and T cell epitopes. Thus, six representative molecules, i.e., Ole e 1, Fra e 1, Sal k 5, Che a 1, Phl p 11 and Pla l 1, were investigated. Endolysosomal degradation and peptide generation were simulated using microsomal fractions of JAWS II dendritic cells. Kinetics and peptide patterns were evaluated by gel electrophoresis and mass spectrometry. In silico MHC (major histocompatibility complex) class II binding prediction was performed with ProPred. Cleavage sites were assigned to the primary and secondary structure, and in silico docking experiments between the protease cathepsin S and Ole e 1 were performed. Different kinetics during endolysosomal degradation were observed while similar peptide profiles especially at the C-termini were detected. Typically, the identified peptide clusters comprised the previously-reported T cell epitopes of Ole e 1, consistent with an in silico analysis of the T cell epitopes. The results emphasize the importance of the fold on allergen processing, as also reflected by conserved cleavage sites located within the large flexible loop. In silico docking and mass spectrometry results suggest that one of the first Ole e 1 cleavages might occur at positions 107–108. Our results provided kinetic and structural information on endolysosomal processing of Ole e 1-like proteins.
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spelling pubmed-55781692017-09-05 Endolysosomal Degradation of Allergenic Ole e 1-Like Proteins: Analysis of Proteolytic Cleavage Sites Revealing T Cell Epitope-Containing Peptides Wildner, Sabrina Elsässer, Brigitta Stemeseder, Teresa Briza, Peter Soh, Wai Tuck Villalba, Mayte Lidholm, Jonas Brandstetter, Hans Gadermaier, Gabriele Int J Mol Sci Article Knowledge of the susceptibility of proteins to endolysosomal proteases provides valuable information on immunogenicity. Though Ole e 1-like proteins are considered relevant allergens, little is known about their immunogenic properties and T cell epitopes. Thus, six representative molecules, i.e., Ole e 1, Fra e 1, Sal k 5, Che a 1, Phl p 11 and Pla l 1, were investigated. Endolysosomal degradation and peptide generation were simulated using microsomal fractions of JAWS II dendritic cells. Kinetics and peptide patterns were evaluated by gel electrophoresis and mass spectrometry. In silico MHC (major histocompatibility complex) class II binding prediction was performed with ProPred. Cleavage sites were assigned to the primary and secondary structure, and in silico docking experiments between the protease cathepsin S and Ole e 1 were performed. Different kinetics during endolysosomal degradation were observed while similar peptide profiles especially at the C-termini were detected. Typically, the identified peptide clusters comprised the previously-reported T cell epitopes of Ole e 1, consistent with an in silico analysis of the T cell epitopes. The results emphasize the importance of the fold on allergen processing, as also reflected by conserved cleavage sites located within the large flexible loop. In silico docking and mass spectrometry results suggest that one of the first Ole e 1 cleavages might occur at positions 107–108. Our results provided kinetic and structural information on endolysosomal processing of Ole e 1-like proteins. MDPI 2017-08-16 /pmc/articles/PMC5578169/ /pubmed/28812992 http://dx.doi.org/10.3390/ijms18081780 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wildner, Sabrina
Elsässer, Brigitta
Stemeseder, Teresa
Briza, Peter
Soh, Wai Tuck
Villalba, Mayte
Lidholm, Jonas
Brandstetter, Hans
Gadermaier, Gabriele
Endolysosomal Degradation of Allergenic Ole e 1-Like Proteins: Analysis of Proteolytic Cleavage Sites Revealing T Cell Epitope-Containing Peptides
title Endolysosomal Degradation of Allergenic Ole e 1-Like Proteins: Analysis of Proteolytic Cleavage Sites Revealing T Cell Epitope-Containing Peptides
title_full Endolysosomal Degradation of Allergenic Ole e 1-Like Proteins: Analysis of Proteolytic Cleavage Sites Revealing T Cell Epitope-Containing Peptides
title_fullStr Endolysosomal Degradation of Allergenic Ole e 1-Like Proteins: Analysis of Proteolytic Cleavage Sites Revealing T Cell Epitope-Containing Peptides
title_full_unstemmed Endolysosomal Degradation of Allergenic Ole e 1-Like Proteins: Analysis of Proteolytic Cleavage Sites Revealing T Cell Epitope-Containing Peptides
title_short Endolysosomal Degradation of Allergenic Ole e 1-Like Proteins: Analysis of Proteolytic Cleavage Sites Revealing T Cell Epitope-Containing Peptides
title_sort endolysosomal degradation of allergenic ole e 1-like proteins: analysis of proteolytic cleavage sites revealing t cell epitope-containing peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578169/
https://www.ncbi.nlm.nih.gov/pubmed/28812992
http://dx.doi.org/10.3390/ijms18081780
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