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Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance

In the present work, a nanostructured ZnO layer was synthesized onto a Metglas magnetoelastic ribbon to immobilize hemoglobin (Hb) on it and study the Hb’s electrochemical behavior towards hydrogen peroxide. Hb oxidation by H(2)O(2) was monitored simultaneously by two different techniques: Cyclic Vo...

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Autores principales: Sagasti, Ariane, Bouropoulos, Nikolaos, Kouzoudis, Dimitris, Panagiotopoulos, Apostolos, Topoglidis, Emmanuel, Gutiérrez, Jon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578215/
https://www.ncbi.nlm.nih.gov/pubmed/28773209
http://dx.doi.org/10.3390/ma10080849
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author Sagasti, Ariane
Bouropoulos, Nikolaos
Kouzoudis, Dimitris
Panagiotopoulos, Apostolos
Topoglidis, Emmanuel
Gutiérrez, Jon
author_facet Sagasti, Ariane
Bouropoulos, Nikolaos
Kouzoudis, Dimitris
Panagiotopoulos, Apostolos
Topoglidis, Emmanuel
Gutiérrez, Jon
author_sort Sagasti, Ariane
collection PubMed
description In the present work, a nanostructured ZnO layer was synthesized onto a Metglas magnetoelastic ribbon to immobilize hemoglobin (Hb) on it and study the Hb’s electrochemical behavior towards hydrogen peroxide. Hb oxidation by H(2)O(2) was monitored simultaneously by two different techniques: Cyclic Voltammetry (CV) and Magnetoelastic Resonance (MR). The Metglas/ZnO/Hb system was simultaneously used as a working electrode for the CV scans and as a magnetoelastic sensor excited by external coils, which drive it to resonance and interrogate it. The ZnO nanoparticles for the ZnO layer were grown hydrothermally and fully characterized by X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and photoluminescence (PL). Additionally, the ZnO layer’s elastic modulus was measured using a new method, which makes use of the Metglas substrate. For the detection experiments, the electrochemical cell was performed with a glass vial, where the three electrodes (working, counter and reference) were immersed into PBS (Phosphate Buffer Solution) solution and small H(2)O(2) drops were added, one at a time. CV scans were taken every 30 s and 5 min after the addition of each drop and meanwhile a magnetoelastic measurement was taken by the external coils. The CV plots reveal direct electrochemical behavior of Hb and display good electrocatalytic response to the reduction of H(2)O(2). The measured catalysis currents increase linearly with the H(2)O(2) concentration in a wide range of 25–350 μM with a correlation coefficient 0.99. The detection limit is 25–50 μM. Moreover, the Metglas/ZnO/Hb electrode displays rapid response (30 s) to H(2)O(2), and exhibits good stability and reproducibility of the measurements. On the other hand, the magnetoelastic measurements show a small linear mass increase versus the H(2)O(2) concentration with a slope of 152 ng/μM, which is probably due to H(2)O(2) adsorption in ZnO during the electrochemical reaction. No such effects were detected during the control experiment when only PBS solution was present for a long time.
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spelling pubmed-55782152017-09-05 Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance Sagasti, Ariane Bouropoulos, Nikolaos Kouzoudis, Dimitris Panagiotopoulos, Apostolos Topoglidis, Emmanuel Gutiérrez, Jon Materials (Basel) Article In the present work, a nanostructured ZnO layer was synthesized onto a Metglas magnetoelastic ribbon to immobilize hemoglobin (Hb) on it and study the Hb’s electrochemical behavior towards hydrogen peroxide. Hb oxidation by H(2)O(2) was monitored simultaneously by two different techniques: Cyclic Voltammetry (CV) and Magnetoelastic Resonance (MR). The Metglas/ZnO/Hb system was simultaneously used as a working electrode for the CV scans and as a magnetoelastic sensor excited by external coils, which drive it to resonance and interrogate it. The ZnO nanoparticles for the ZnO layer were grown hydrothermally and fully characterized by X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and photoluminescence (PL). Additionally, the ZnO layer’s elastic modulus was measured using a new method, which makes use of the Metglas substrate. For the detection experiments, the electrochemical cell was performed with a glass vial, where the three electrodes (working, counter and reference) were immersed into PBS (Phosphate Buffer Solution) solution and small H(2)O(2) drops were added, one at a time. CV scans were taken every 30 s and 5 min after the addition of each drop and meanwhile a magnetoelastic measurement was taken by the external coils. The CV plots reveal direct electrochemical behavior of Hb and display good electrocatalytic response to the reduction of H(2)O(2). The measured catalysis currents increase linearly with the H(2)O(2) concentration in a wide range of 25–350 μM with a correlation coefficient 0.99. The detection limit is 25–50 μM. Moreover, the Metglas/ZnO/Hb electrode displays rapid response (30 s) to H(2)O(2), and exhibits good stability and reproducibility of the measurements. On the other hand, the magnetoelastic measurements show a small linear mass increase versus the H(2)O(2) concentration with a slope of 152 ng/μM, which is probably due to H(2)O(2) adsorption in ZnO during the electrochemical reaction. No such effects were detected during the control experiment when only PBS solution was present for a long time. MDPI 2017-07-25 /pmc/articles/PMC5578215/ /pubmed/28773209 http://dx.doi.org/10.3390/ma10080849 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sagasti, Ariane
Bouropoulos, Nikolaos
Kouzoudis, Dimitris
Panagiotopoulos, Apostolos
Topoglidis, Emmanuel
Gutiérrez, Jon
Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance
title Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance
title_full Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance
title_fullStr Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance
title_full_unstemmed Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance
title_short Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance
title_sort nanostructured zno in a metglas/zno/hemoglobin modified electrode to detect the oxidation of the hemoglobin simultaneously by cyclic voltammetry and magnetoelastic resonance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578215/
https://www.ncbi.nlm.nih.gov/pubmed/28773209
http://dx.doi.org/10.3390/ma10080849
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