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Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration

In this study, we investigated the effect of supplementing a non-dispersive dicalcium phosphate-rich calcium phosphate bone cement (DCP-rich CPC) with type I collagen on in vitro cellular activities and its performance as a bone graft material. Varying amounts of type I collagen were added during th...

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Autores principales: Hu, Ming-Hsien, Lee, Pei-Yuan, Chen, Wen-Cheng, Hu, Jin-Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578276/
https://www.ncbi.nlm.nih.gov/pubmed/28783082
http://dx.doi.org/10.3390/ma10080910
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author Hu, Ming-Hsien
Lee, Pei-Yuan
Chen, Wen-Cheng
Hu, Jin-Jia
author_facet Hu, Ming-Hsien
Lee, Pei-Yuan
Chen, Wen-Cheng
Hu, Jin-Jia
author_sort Hu, Ming-Hsien
collection PubMed
description In this study, we investigated the effect of supplementing a non-dispersive dicalcium phosphate-rich calcium phosphate bone cement (DCP-rich CPC) with type I collagen on in vitro cellular activities and its performance as a bone graft material. Varying amounts of type I collagen were added during the preparation of the DCP-rich CPC. In vitro cell adhesion, morphology, viability, and alkaline phosphatase (ALP) activity were evaluated using progenitor bone cells. Bone graft performance was evaluated via a rat posterolateral lumbar fusion model and osteointegration of the implant. New bone formations in the restorative sites were assessed by micro-computed tomography (micro-CT) and histological analysis. We found that the incorporation of collagen into the DCP-rich CPC was associated with increased cell adhesion, cell viability, and ALP activity in vitro. The spinal fusion model revealed a significant increase in bone regeneration. Additionally, better osseointegration was observed between the host bone and graft with the DCP-rich CPC supplemented with collagen than with the collagen-free DCP-rich CPC control graft. Furthermore, compared to the control graft, the results of micro-CT showed that a smaller amount of residual material was observed with the collagen-containing DCP-rich CPC graft compared with the control graft, which suggests the collagen supplement enhanced new bone formation. Of the different mixtures evaluated in this study (0.8 g DCP-rich CPC supplemented with 0.1, 0.2, and 0.4 mL type I collagen, respectively), DCP-rich CPC supplemented with 0.4 mL collagen led to the highest level of osteogenesis. Our results suggest that the DCP-rich CPC supplemented with collagen has potential to be used as an effective bone graft material in spinal surgery.
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spelling pubmed-55782762017-09-05 Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration Hu, Ming-Hsien Lee, Pei-Yuan Chen, Wen-Cheng Hu, Jin-Jia Materials (Basel) Article In this study, we investigated the effect of supplementing a non-dispersive dicalcium phosphate-rich calcium phosphate bone cement (DCP-rich CPC) with type I collagen on in vitro cellular activities and its performance as a bone graft material. Varying amounts of type I collagen were added during the preparation of the DCP-rich CPC. In vitro cell adhesion, morphology, viability, and alkaline phosphatase (ALP) activity were evaluated using progenitor bone cells. Bone graft performance was evaluated via a rat posterolateral lumbar fusion model and osteointegration of the implant. New bone formations in the restorative sites were assessed by micro-computed tomography (micro-CT) and histological analysis. We found that the incorporation of collagen into the DCP-rich CPC was associated with increased cell adhesion, cell viability, and ALP activity in vitro. The spinal fusion model revealed a significant increase in bone regeneration. Additionally, better osseointegration was observed between the host bone and graft with the DCP-rich CPC supplemented with collagen than with the collagen-free DCP-rich CPC control graft. Furthermore, compared to the control graft, the results of micro-CT showed that a smaller amount of residual material was observed with the collagen-containing DCP-rich CPC graft compared with the control graft, which suggests the collagen supplement enhanced new bone formation. Of the different mixtures evaluated in this study (0.8 g DCP-rich CPC supplemented with 0.1, 0.2, and 0.4 mL type I collagen, respectively), DCP-rich CPC supplemented with 0.4 mL collagen led to the highest level of osteogenesis. Our results suggest that the DCP-rich CPC supplemented with collagen has potential to be used as an effective bone graft material in spinal surgery. MDPI 2017-08-06 /pmc/articles/PMC5578276/ /pubmed/28783082 http://dx.doi.org/10.3390/ma10080910 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hu, Ming-Hsien
Lee, Pei-Yuan
Chen, Wen-Cheng
Hu, Jin-Jia
Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration
title Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration
title_full Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration
title_fullStr Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration
title_full_unstemmed Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration
title_short Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration
title_sort incorporation of collagen in calcium phosphate cements for controlling osseointegration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578276/
https://www.ncbi.nlm.nih.gov/pubmed/28783082
http://dx.doi.org/10.3390/ma10080910
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