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Uncovering unseen fungal diversity from plant DNA banks

Throughout the world DNA banks are used as storage repositories for genetic diversity of organisms ranging from plants to insects to mammals. Designed to preserve the genetic information for organisms of interest, these banks also indirectly preserve organisms’ associated microbiomes, including fung...

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Detalles Bibliográficos
Autores principales: Datlof, Erin M., Amend, Anthony S., Earl, Kamala, Hayward, Jeremy, Morden, Clifford W., Wade, Rachael, Zahn, Geoffrey, Hynson, Nicole A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578370/
https://www.ncbi.nlm.nih.gov/pubmed/28875077
http://dx.doi.org/10.7717/peerj.3730
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author Datlof, Erin M.
Amend, Anthony S.
Earl, Kamala
Hayward, Jeremy
Morden, Clifford W.
Wade, Rachael
Zahn, Geoffrey
Hynson, Nicole A.
author_facet Datlof, Erin M.
Amend, Anthony S.
Earl, Kamala
Hayward, Jeremy
Morden, Clifford W.
Wade, Rachael
Zahn, Geoffrey
Hynson, Nicole A.
author_sort Datlof, Erin M.
collection PubMed
description Throughout the world DNA banks are used as storage repositories for genetic diversity of organisms ranging from plants to insects to mammals. Designed to preserve the genetic information for organisms of interest, these banks also indirectly preserve organisms’ associated microbiomes, including fungi associated with plant tissues. Studies of fungal biodiversity lag far behind those of macroorganisms, such as plants, and estimates of global fungal richness are still widely debated. Utilizing previously collected specimens to study patterns of fungal diversity could significantly increase our understanding of overall patterns of biodiversity from snapshots in time. Here, we investigated the fungi inhabiting the phylloplane among species of the endemic Hawaiian plant genus, Clermontia (Campanulaceae). Utilizing next generation DNA amplicon sequencing, we uncovered approximately 1,780 fungal operational taxonomic units from just 20 DNA bank samples collected throughout the main Hawaiian Islands. Using these historical samples, we tested the macroecological pattern of decreasing community similarity with decreasing geographic proximity. We found a significant distance decay pattern among Clermontia associated fungal communities. This study provides the first insights into elucidating patterns of microbial diversity through the use of DNA bank repository samples.
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spelling pubmed-55783702017-09-05 Uncovering unseen fungal diversity from plant DNA banks Datlof, Erin M. Amend, Anthony S. Earl, Kamala Hayward, Jeremy Morden, Clifford W. Wade, Rachael Zahn, Geoffrey Hynson, Nicole A. PeerJ Biodiversity Throughout the world DNA banks are used as storage repositories for genetic diversity of organisms ranging from plants to insects to mammals. Designed to preserve the genetic information for organisms of interest, these banks also indirectly preserve organisms’ associated microbiomes, including fungi associated with plant tissues. Studies of fungal biodiversity lag far behind those of macroorganisms, such as plants, and estimates of global fungal richness are still widely debated. Utilizing previously collected specimens to study patterns of fungal diversity could significantly increase our understanding of overall patterns of biodiversity from snapshots in time. Here, we investigated the fungi inhabiting the phylloplane among species of the endemic Hawaiian plant genus, Clermontia (Campanulaceae). Utilizing next generation DNA amplicon sequencing, we uncovered approximately 1,780 fungal operational taxonomic units from just 20 DNA bank samples collected throughout the main Hawaiian Islands. Using these historical samples, we tested the macroecological pattern of decreasing community similarity with decreasing geographic proximity. We found a significant distance decay pattern among Clermontia associated fungal communities. This study provides the first insights into elucidating patterns of microbial diversity through the use of DNA bank repository samples. PeerJ Inc. 2017-08-28 /pmc/articles/PMC5578370/ /pubmed/28875077 http://dx.doi.org/10.7717/peerj.3730 Text en ©2017 Datlof et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biodiversity
Datlof, Erin M.
Amend, Anthony S.
Earl, Kamala
Hayward, Jeremy
Morden, Clifford W.
Wade, Rachael
Zahn, Geoffrey
Hynson, Nicole A.
Uncovering unseen fungal diversity from plant DNA banks
title Uncovering unseen fungal diversity from plant DNA banks
title_full Uncovering unseen fungal diversity from plant DNA banks
title_fullStr Uncovering unseen fungal diversity from plant DNA banks
title_full_unstemmed Uncovering unseen fungal diversity from plant DNA banks
title_short Uncovering unseen fungal diversity from plant DNA banks
title_sort uncovering unseen fungal diversity from plant dna banks
topic Biodiversity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578370/
https://www.ncbi.nlm.nih.gov/pubmed/28875077
http://dx.doi.org/10.7717/peerj.3730
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