A non-destructive DNA sampling technique for herbarium specimens

Herbarium specimens are an important source of DNA for plant research but current sampling methods require the removal of material for DNA extraction. This is undesirable for irreplaceable specimens such as rare species or type material. Here I present the first non-destructive sampling method for e...

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Detalles Bibliográficos
Autor principal: Shepherd, Lara D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578499/
https://www.ncbi.nlm.nih.gov/pubmed/28859137
http://dx.doi.org/10.1371/journal.pone.0183555
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author Shepherd, Lara D.
author_facet Shepherd, Lara D.
author_sort Shepherd, Lara D.
collection PubMed
description Herbarium specimens are an important source of DNA for plant research but current sampling methods require the removal of material for DNA extraction. This is undesirable for irreplaceable specimens such as rare species or type material. Here I present the first non-destructive sampling method for extracting DNA from herbarium specimens. DNA was successfully retrieved from robust leaves and/or stems of herbarium specimens up to 73 years old.
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spelling pubmed-55784992017-09-15 A non-destructive DNA sampling technique for herbarium specimens Shepherd, Lara D. PLoS One Research Article Herbarium specimens are an important source of DNA for plant research but current sampling methods require the removal of material for DNA extraction. This is undesirable for irreplaceable specimens such as rare species or type material. Here I present the first non-destructive sampling method for extracting DNA from herbarium specimens. DNA was successfully retrieved from robust leaves and/or stems of herbarium specimens up to 73 years old. Public Library of Science 2017-08-31 /pmc/articles/PMC5578499/ /pubmed/28859137 http://dx.doi.org/10.1371/journal.pone.0183555 Text en © 2017 Lara D. Shepherd http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shepherd, Lara D.
A non-destructive DNA sampling technique for herbarium specimens
title A non-destructive DNA sampling technique for herbarium specimens
title_full A non-destructive DNA sampling technique for herbarium specimens
title_fullStr A non-destructive DNA sampling technique for herbarium specimens
title_full_unstemmed A non-destructive DNA sampling technique for herbarium specimens
title_short A non-destructive DNA sampling technique for herbarium specimens
title_sort non-destructive dna sampling technique for herbarium specimens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578499/
https://www.ncbi.nlm.nih.gov/pubmed/28859137
http://dx.doi.org/10.1371/journal.pone.0183555
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