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Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp.
To identify putative cis-elements involved in gene expression regulation in Leishmania, we previously conducted an in silico investigation to find conserved intercoding sequences (CICS) in the genomes of L. major, L. infantum, and L. braziliensis. Here, the CICS databank was explored to search for s...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578504/ https://www.ncbi.nlm.nih.gov/pubmed/28859096 http://dx.doi.org/10.1371/journal.pone.0183401 |
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author | Terrão, Monica Cristina Rosas de Vasconcelos, Elton José Defina, Tânia Aquino Myler, Peter J. Cruz, Angela Kaysel |
author_facet | Terrão, Monica Cristina Rosas de Vasconcelos, Elton José Defina, Tânia Aquino Myler, Peter J. Cruz, Angela Kaysel |
author_sort | Terrão, Monica Cristina |
collection | PubMed |
description | To identify putative cis-elements involved in gene expression regulation in Leishmania, we previously conducted an in silico investigation to find conserved intercoding sequences (CICS) in the genomes of L. major, L. infantum, and L. braziliensis. Here, the CICS databank was explored to search for sequences that were present in the untranslated regions (UTRs) of groups of genes showing similar expression profiles during in vitro differentiation. Using a selectable marker as a reporter gene, flanked by either an intact 3' UTR or a UTR lacking the conserved element, the regulatory role of a CICS was confirmed. We observed that the pattern of modulation of the mRNA levels was altered in the absence of the CICS. We also identified putative CICS RNA-binding proteins. This study suggests that the publicly available CICS database is a useful tool for identifying regulatory cis-elements for Leishmania genes and suggests the existence of post-transcriptional regulons in Leishmania. |
format | Online Article Text |
id | pubmed-5578504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-55785042017-09-15 Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp. Terrão, Monica Cristina Rosas de Vasconcelos, Elton José Defina, Tânia Aquino Myler, Peter J. Cruz, Angela Kaysel PLoS One Research Article To identify putative cis-elements involved in gene expression regulation in Leishmania, we previously conducted an in silico investigation to find conserved intercoding sequences (CICS) in the genomes of L. major, L. infantum, and L. braziliensis. Here, the CICS databank was explored to search for sequences that were present in the untranslated regions (UTRs) of groups of genes showing similar expression profiles during in vitro differentiation. Using a selectable marker as a reporter gene, flanked by either an intact 3' UTR or a UTR lacking the conserved element, the regulatory role of a CICS was confirmed. We observed that the pattern of modulation of the mRNA levels was altered in the absence of the CICS. We also identified putative CICS RNA-binding proteins. This study suggests that the publicly available CICS database is a useful tool for identifying regulatory cis-elements for Leishmania genes and suggests the existence of post-transcriptional regulons in Leishmania. Public Library of Science 2017-08-31 /pmc/articles/PMC5578504/ /pubmed/28859096 http://dx.doi.org/10.1371/journal.pone.0183401 Text en © 2017 Terrão et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Terrão, Monica Cristina Rosas de Vasconcelos, Elton José Defina, Tânia Aquino Myler, Peter J. Cruz, Angela Kaysel Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp. |
title | Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp. |
title_full | Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp. |
title_fullStr | Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp. |
title_full_unstemmed | Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp. |
title_short | Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp. |
title_sort | disclosing 3' utr cis-elements and putative partners involved in gene expression regulation in leishmania spp. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578504/ https://www.ncbi.nlm.nih.gov/pubmed/28859096 http://dx.doi.org/10.1371/journal.pone.0183401 |
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