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Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp.

To identify putative cis-elements involved in gene expression regulation in Leishmania, we previously conducted an in silico investigation to find conserved intercoding sequences (CICS) in the genomes of L. major, L. infantum, and L. braziliensis. Here, the CICS databank was explored to search for s...

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Autores principales: Terrão, Monica Cristina, Rosas de Vasconcelos, Elton José, Defina, Tânia Aquino, Myler, Peter J., Cruz, Angela Kaysel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578504/
https://www.ncbi.nlm.nih.gov/pubmed/28859096
http://dx.doi.org/10.1371/journal.pone.0183401
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author Terrão, Monica Cristina
Rosas de Vasconcelos, Elton José
Defina, Tânia Aquino
Myler, Peter J.
Cruz, Angela Kaysel
author_facet Terrão, Monica Cristina
Rosas de Vasconcelos, Elton José
Defina, Tânia Aquino
Myler, Peter J.
Cruz, Angela Kaysel
author_sort Terrão, Monica Cristina
collection PubMed
description To identify putative cis-elements involved in gene expression regulation in Leishmania, we previously conducted an in silico investigation to find conserved intercoding sequences (CICS) in the genomes of L. major, L. infantum, and L. braziliensis. Here, the CICS databank was explored to search for sequences that were present in the untranslated regions (UTRs) of groups of genes showing similar expression profiles during in vitro differentiation. Using a selectable marker as a reporter gene, flanked by either an intact 3' UTR or a UTR lacking the conserved element, the regulatory role of a CICS was confirmed. We observed that the pattern of modulation of the mRNA levels was altered in the absence of the CICS. We also identified putative CICS RNA-binding proteins. This study suggests that the publicly available CICS database is a useful tool for identifying regulatory cis-elements for Leishmania genes and suggests the existence of post-transcriptional regulons in Leishmania.
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spelling pubmed-55785042017-09-15 Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp. Terrão, Monica Cristina Rosas de Vasconcelos, Elton José Defina, Tânia Aquino Myler, Peter J. Cruz, Angela Kaysel PLoS One Research Article To identify putative cis-elements involved in gene expression regulation in Leishmania, we previously conducted an in silico investigation to find conserved intercoding sequences (CICS) in the genomes of L. major, L. infantum, and L. braziliensis. Here, the CICS databank was explored to search for sequences that were present in the untranslated regions (UTRs) of groups of genes showing similar expression profiles during in vitro differentiation. Using a selectable marker as a reporter gene, flanked by either an intact 3' UTR or a UTR lacking the conserved element, the regulatory role of a CICS was confirmed. We observed that the pattern of modulation of the mRNA levels was altered in the absence of the CICS. We also identified putative CICS RNA-binding proteins. This study suggests that the publicly available CICS database is a useful tool for identifying regulatory cis-elements for Leishmania genes and suggests the existence of post-transcriptional regulons in Leishmania. Public Library of Science 2017-08-31 /pmc/articles/PMC5578504/ /pubmed/28859096 http://dx.doi.org/10.1371/journal.pone.0183401 Text en © 2017 Terrão et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Terrão, Monica Cristina
Rosas de Vasconcelos, Elton José
Defina, Tânia Aquino
Myler, Peter J.
Cruz, Angela Kaysel
Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp.
title Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp.
title_full Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp.
title_fullStr Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp.
title_full_unstemmed Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp.
title_short Disclosing 3' UTR cis-elements and putative partners involved in gene expression regulation in Leishmania spp.
title_sort disclosing 3' utr cis-elements and putative partners involved in gene expression regulation in leishmania spp.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578504/
https://www.ncbi.nlm.nih.gov/pubmed/28859096
http://dx.doi.org/10.1371/journal.pone.0183401
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