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Exacerbation of colon carcinogenesis by Blastocystis sp.
Colorectal cancer (CRC) is one the most commonly diagnosed cancers worldwide and the number is increasing every year. Despite advances in screening programs, CRC remains as the second leading cause of cancer deaths in the United States. Oxidative stress plays an important role in the molecular mecha...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578604/ https://www.ncbi.nlm.nih.gov/pubmed/28859095 http://dx.doi.org/10.1371/journal.pone.0183097 |
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author | Kumarasamy, Vinoth Kuppusamy, Umah Rani Jayalakshmi, Pailoor Samudi, Chandramathi Ragavan, Nanthiney Devi Kumar, Suresh |
author_facet | Kumarasamy, Vinoth Kuppusamy, Umah Rani Jayalakshmi, Pailoor Samudi, Chandramathi Ragavan, Nanthiney Devi Kumar, Suresh |
author_sort | Kumarasamy, Vinoth |
collection | PubMed |
description | Colorectal cancer (CRC) is one the most commonly diagnosed cancers worldwide and the number is increasing every year. Despite advances in screening programs, CRC remains as the second leading cause of cancer deaths in the United States. Oxidative stress plays an important role in the molecular mechanisms of colorectal cancer (CRC) and has been shown to be associated with Blastocystis sp., a common intestinal microorganism. In the present study, we aimed to identify a role for Blastocystis sp. in exacerbating carcinogenesis using in vivo rat model. Methylene blue staining was used to identify colonic aberrant crypt foci (ACF) and adenomas formation in infected rats whilst elevation of oxidative stress biomarker levels in the urine and serum samples were evaluated using biochemical assays. Histological changes of the intestinal mucosa were observed and a significant number of ACF was found in Blastocystis sp. infected AOM-rats compared to the AOM-controls. High levels of urinary oxidative indices including advanced oxidative protein products (AOPP) and hydrogen peroxide were observed in Blastocystis sp. infected AOM-rats compared to the uninfected AOM-rats. Our study provides evidence that Blastocystis sp. has a significant role in enhancing AOM-induced carcinogenesis by resulting damage to the intestinal epithelium and promoting oxidative damage in Blastocystis sp. infected rats. |
format | Online Article Text |
id | pubmed-5578604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-55786042017-09-15 Exacerbation of colon carcinogenesis by Blastocystis sp. Kumarasamy, Vinoth Kuppusamy, Umah Rani Jayalakshmi, Pailoor Samudi, Chandramathi Ragavan, Nanthiney Devi Kumar, Suresh PLoS One Research Article Colorectal cancer (CRC) is one the most commonly diagnosed cancers worldwide and the number is increasing every year. Despite advances in screening programs, CRC remains as the second leading cause of cancer deaths in the United States. Oxidative stress plays an important role in the molecular mechanisms of colorectal cancer (CRC) and has been shown to be associated with Blastocystis sp., a common intestinal microorganism. In the present study, we aimed to identify a role for Blastocystis sp. in exacerbating carcinogenesis using in vivo rat model. Methylene blue staining was used to identify colonic aberrant crypt foci (ACF) and adenomas formation in infected rats whilst elevation of oxidative stress biomarker levels in the urine and serum samples were evaluated using biochemical assays. Histological changes of the intestinal mucosa were observed and a significant number of ACF was found in Blastocystis sp. infected AOM-rats compared to the AOM-controls. High levels of urinary oxidative indices including advanced oxidative protein products (AOPP) and hydrogen peroxide were observed in Blastocystis sp. infected AOM-rats compared to the uninfected AOM-rats. Our study provides evidence that Blastocystis sp. has a significant role in enhancing AOM-induced carcinogenesis by resulting damage to the intestinal epithelium and promoting oxidative damage in Blastocystis sp. infected rats. Public Library of Science 2017-08-31 /pmc/articles/PMC5578604/ /pubmed/28859095 http://dx.doi.org/10.1371/journal.pone.0183097 Text en © 2017 Kumarasamy et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kumarasamy, Vinoth Kuppusamy, Umah Rani Jayalakshmi, Pailoor Samudi, Chandramathi Ragavan, Nanthiney Devi Kumar, Suresh Exacerbation of colon carcinogenesis by Blastocystis sp. |
title | Exacerbation of colon carcinogenesis by Blastocystis sp. |
title_full | Exacerbation of colon carcinogenesis by Blastocystis sp. |
title_fullStr | Exacerbation of colon carcinogenesis by Blastocystis sp. |
title_full_unstemmed | Exacerbation of colon carcinogenesis by Blastocystis sp. |
title_short | Exacerbation of colon carcinogenesis by Blastocystis sp. |
title_sort | exacerbation of colon carcinogenesis by blastocystis sp. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578604/ https://www.ncbi.nlm.nih.gov/pubmed/28859095 http://dx.doi.org/10.1371/journal.pone.0183097 |
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