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Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development
An ‘interactome’ screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new m...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578738/ https://www.ncbi.nlm.nih.gov/pubmed/28829740 http://dx.doi.org/10.7554/eLife.28111 |
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author | Li, Hanqing Watson, Ash Olechwier, Agnieszka Anaya, Michael Sorooshyari, Siamak K Harnett, Dermott P Lee, Hyung-Kook (Peter) Vielmetter, Jost Fares, Mario A Garcia, K Christopher Özkan, Engin Labrador, Juan-Pablo Zinn, Kai |
author_facet | Li, Hanqing Watson, Ash Olechwier, Agnieszka Anaya, Michael Sorooshyari, Siamak K Harnett, Dermott P Lee, Hyung-Kook (Peter) Vielmetter, Jost Fares, Mario A Garcia, K Christopher Özkan, Engin Labrador, Juan-Pablo Zinn, Kai |
author_sort | Li, Hanqing |
collection | PubMed |
description | An ‘interactome’ screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we ‘deorphanized’ four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons. |
format | Online Article Text |
id | pubmed-5578738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-55787382017-09-01 Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development Li, Hanqing Watson, Ash Olechwier, Agnieszka Anaya, Michael Sorooshyari, Siamak K Harnett, Dermott P Lee, Hyung-Kook (Peter) Vielmetter, Jost Fares, Mario A Garcia, K Christopher Özkan, Engin Labrador, Juan-Pablo Zinn, Kai eLife Neuroscience An ‘interactome’ screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we ‘deorphanized’ four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons. eLife Sciences Publications, Ltd 2017-08-15 /pmc/articles/PMC5578738/ /pubmed/28829740 http://dx.doi.org/10.7554/eLife.28111 Text en © 2017, Li et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Li, Hanqing Watson, Ash Olechwier, Agnieszka Anaya, Michael Sorooshyari, Siamak K Harnett, Dermott P Lee, Hyung-Kook (Peter) Vielmetter, Jost Fares, Mario A Garcia, K Christopher Özkan, Engin Labrador, Juan-Pablo Zinn, Kai Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development |
title | Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development |
title_full | Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development |
title_fullStr | Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development |
title_full_unstemmed | Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development |
title_short | Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development |
title_sort | deconstruction of the beaten path-sidestep interaction network provides insights into neuromuscular system development |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578738/ https://www.ncbi.nlm.nih.gov/pubmed/28829740 http://dx.doi.org/10.7554/eLife.28111 |
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