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Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development

An ‘interactome’ screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new m...

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Autores principales: Li, Hanqing, Watson, Ash, Olechwier, Agnieszka, Anaya, Michael, Sorooshyari, Siamak K, Harnett, Dermott P, Lee, Hyung-Kook (Peter), Vielmetter, Jost, Fares, Mario A, Garcia, K Christopher, Özkan, Engin, Labrador, Juan-Pablo, Zinn, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578738/
https://www.ncbi.nlm.nih.gov/pubmed/28829740
http://dx.doi.org/10.7554/eLife.28111
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author Li, Hanqing
Watson, Ash
Olechwier, Agnieszka
Anaya, Michael
Sorooshyari, Siamak K
Harnett, Dermott P
Lee, Hyung-Kook (Peter)
Vielmetter, Jost
Fares, Mario A
Garcia, K Christopher
Özkan, Engin
Labrador, Juan-Pablo
Zinn, Kai
author_facet Li, Hanqing
Watson, Ash
Olechwier, Agnieszka
Anaya, Michael
Sorooshyari, Siamak K
Harnett, Dermott P
Lee, Hyung-Kook (Peter)
Vielmetter, Jost
Fares, Mario A
Garcia, K Christopher
Özkan, Engin
Labrador, Juan-Pablo
Zinn, Kai
author_sort Li, Hanqing
collection PubMed
description An ‘interactome’ screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we ‘deorphanized’ four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons.
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spelling pubmed-55787382017-09-01 Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development Li, Hanqing Watson, Ash Olechwier, Agnieszka Anaya, Michael Sorooshyari, Siamak K Harnett, Dermott P Lee, Hyung-Kook (Peter) Vielmetter, Jost Fares, Mario A Garcia, K Christopher Özkan, Engin Labrador, Juan-Pablo Zinn, Kai eLife Neuroscience An ‘interactome’ screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we ‘deorphanized’ four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons. eLife Sciences Publications, Ltd 2017-08-15 /pmc/articles/PMC5578738/ /pubmed/28829740 http://dx.doi.org/10.7554/eLife.28111 Text en © 2017, Li et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Li, Hanqing
Watson, Ash
Olechwier, Agnieszka
Anaya, Michael
Sorooshyari, Siamak K
Harnett, Dermott P
Lee, Hyung-Kook (Peter)
Vielmetter, Jost
Fares, Mario A
Garcia, K Christopher
Özkan, Engin
Labrador, Juan-Pablo
Zinn, Kai
Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development
title Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development
title_full Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development
title_fullStr Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development
title_full_unstemmed Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development
title_short Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development
title_sort deconstruction of the beaten path-sidestep interaction network provides insights into neuromuscular system development
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578738/
https://www.ncbi.nlm.nih.gov/pubmed/28829740
http://dx.doi.org/10.7554/eLife.28111
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