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Cardiac optogenetics: using light to monitor cardiac physiology

Our current understanding of cardiac excitation and its coupling to contraction is largely based on ex vivo studies utilising fluorescent organic dyes to assess cardiac action potentials and signal transduction. Recent advances in optogenetic sensors open exciting new possibilities for cardiac resea...

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Autores principales: Koopman, Charlotte D., Zimmermann, Wolfram H., Knöpfel, Thomas, de Boer, Teun P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5579185/
https://www.ncbi.nlm.nih.gov/pubmed/28861604
http://dx.doi.org/10.1007/s00395-017-0645-y
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author Koopman, Charlotte D.
Zimmermann, Wolfram H.
Knöpfel, Thomas
de Boer, Teun P.
author_facet Koopman, Charlotte D.
Zimmermann, Wolfram H.
Knöpfel, Thomas
de Boer, Teun P.
author_sort Koopman, Charlotte D.
collection PubMed
description Our current understanding of cardiac excitation and its coupling to contraction is largely based on ex vivo studies utilising fluorescent organic dyes to assess cardiac action potentials and signal transduction. Recent advances in optogenetic sensors open exciting new possibilities for cardiac research and allow us to answer research questions that cannot be addressed using the classic organic dyes. Especially thrilling is the possibility to use optogenetic sensors to record parameters of cardiac excitation and contraction in vivo. In addition, optogenetics provide a high spatial resolution, as sensors can be coupled to motifs and targeted to specific cell types and subcellular domains of the heart. In this review, we will give a comprehensive overview of relevant optogenetic sensors, how they can be utilised in cardiac research and how they have been applied in cardiac research up to now.
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spelling pubmed-55791852017-09-26 Cardiac optogenetics: using light to monitor cardiac physiology Koopman, Charlotte D. Zimmermann, Wolfram H. Knöpfel, Thomas de Boer, Teun P. Basic Res Cardiol Review Our current understanding of cardiac excitation and its coupling to contraction is largely based on ex vivo studies utilising fluorescent organic dyes to assess cardiac action potentials and signal transduction. Recent advances in optogenetic sensors open exciting new possibilities for cardiac research and allow us to answer research questions that cannot be addressed using the classic organic dyes. Especially thrilling is the possibility to use optogenetic sensors to record parameters of cardiac excitation and contraction in vivo. In addition, optogenetics provide a high spatial resolution, as sensors can be coupled to motifs and targeted to specific cell types and subcellular domains of the heart. In this review, we will give a comprehensive overview of relevant optogenetic sensors, how they can be utilised in cardiac research and how they have been applied in cardiac research up to now. Springer Berlin Heidelberg 2017-08-31 2017 /pmc/articles/PMC5579185/ /pubmed/28861604 http://dx.doi.org/10.1007/s00395-017-0645-y Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Review
Koopman, Charlotte D.
Zimmermann, Wolfram H.
Knöpfel, Thomas
de Boer, Teun P.
Cardiac optogenetics: using light to monitor cardiac physiology
title Cardiac optogenetics: using light to monitor cardiac physiology
title_full Cardiac optogenetics: using light to monitor cardiac physiology
title_fullStr Cardiac optogenetics: using light to monitor cardiac physiology
title_full_unstemmed Cardiac optogenetics: using light to monitor cardiac physiology
title_short Cardiac optogenetics: using light to monitor cardiac physiology
title_sort cardiac optogenetics: using light to monitor cardiac physiology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5579185/
https://www.ncbi.nlm.nih.gov/pubmed/28861604
http://dx.doi.org/10.1007/s00395-017-0645-y
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