Cargando…

Expression and purification of untagged GlnK proteins from actinobacteria

The PII protein family constitutes one of the most conserved and well distributed family of signal transduction proteins in nature. These proteins play key roles in nitrogen and carbon metabolism. PII function has been well documented in Gram-negative bacteria. However, there are very few reports de...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gerhardt, Edileusa C.M., Moure, Vivian R., Souza, Andrey W., Pedrosa, Fabio O., Souza, Emanuel M., Diacovich, Lautaro, Gramajo, Hugo, Huergo, Luciano F.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Leibniz Research Centre for Working Environment and Human Factors 2017
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5579400/ https://www.ncbi.nlm.nih.gov/pubmed/28900375 http://dx.doi.org/10.17179/excli2017-394

Ejemplares similares

Association and dissociation of the GlnK–AmtB complex in response to cellular nitrogen status can occur in the absence of GlnK post-translational modification
por: Radchenko, Martha V., et al.
Publicado: (2014)

GlnK Facilitates the Dynamic Regulation of Bacterial Nitrogen Assimilation
por: Gosztolai, Adam, et al.
Publicado: (2017)

Interaction of the Nitrogen Regulatory Protein GlnB (P(II)) with Biotin Carboxyl Carrier Protein (BCCP) Controls Acetyl-CoA Levels in the Cyanobacterium Synechocystis sp. PCC 6803
por: Hauf, Waldemar, et al.
Publicado: (2016)

Mechanism of Disruption of the Amt-GlnK Complex by P(II)-Mediated Sensing of 2-Oxoglutarate
por: Maier, Sarah, et al.
Publicado: (2011)

Crystal structures of adenylylated and unadenylylated P(II) protein GlnK from Corynebacterium glutamicum
por: Grau, Florian C., et al.
Publicado: (2021)

Bacillus velezensis LG37: transcriptome profiling and functional verification of GlnK and MnrA in ammonia assimilation
por: Liu, Guangxin, et al.
Publicado: (2020)

M. mazei glutamine synthetase and glutamine synthetase-GlnK1 structures reveal enzyme regulation by oligomer modulation
por: Schumacher, Maria A., et al.
Publicado: (2023)

Nitrogen regulation of protein–protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea
por: Pedro-Roig, Laia, et al.
Publicado: (2013)

Pleiotropic Effect of AccD5 and AccE5 Depletion in Acyl-Coenzyme A Carboxylase Activity and in Lipid Biosynthesis in Mycobacteria
por: Bazet Lyonnet, Bernardo, et al.
Publicado: (2014)

3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection
por: Tomassetti, Mauro, et al.
Publicado: (2018)

Correction: Pleiotropic Effect of AccD5 and AccE5 Depletion in Acyl-Coenzyme A Carboxylase Activity and in Lipid Biosynthesis in Mycobacteria
por: Lyonnet, Bernardo Bazet, et al.
Publicado: (2020)

Structural insights into the transcription activation mechanism of the global regulator GlnR from actinobacteria
por: Shi, Jing, et al.
Publicado: (2023)

Adenylylation of mycobacterial Glnk (PII) protein is induced by nitrogen limitation
por: Williams, Kerstin J., et al.
Publicado: (2013)

Mycobacterium tuberculosis FasR senses long fatty acyl-CoA through a tunnel and a hydrophobic transmission spine
por: Lara, Julia, et al.
Publicado: (2020)

Effect of ATP and 2-oxoglutarate on the in vitro interaction between the NifA GAF domain and the GlnB protein of Azospirillum brasilense
por: Sotomaior, P., et al.
Publicado: (2012)

NAD(+) biosynthesis in bacteria is controlled by global carbon/nitrogen levels via PII signaling
por: Santos, Adrian Richard Schenberger, et al.
Publicado: (2020)

Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum
por: Habibi Arejan, Neda, et al.
Publicado: (2023)

ClearColi as a platform for untagged pneumococcal surface protein A production: cultivation strategy, bioreactor culture, and purification
por: Cardoso, Valdemir M., et al.
Publicado: (2022)

The Protein-Protein Interaction Network Reveals a Novel Role of the Signal Transduction Protein PII in the Control of c-di-GMP Homeostasis in Azospirillum brasilense
por: Gerhardt, Edileusa C. M., et al.
Publicado: (2020)

Measurement of $\eta_{c}$ production in untagged two-photon collisions at LEP
por: Adriani, O, et al.
Publicado: (1993)

Untagged Analysis of B0s -> J/psi phi Channel
por: Charles, François
Publicado: (1999)

Production of e, $\mu$ and $\tau$ Pairs in Untagged Two-Photon Collisions at LEP
por: Acciarri, M, et al.
Publicado: (1997)

Streamlined, automated protocols for the production of milligram quantities of untagged recombinant human cyclophilin-A (hCypA) and untagged human proliferating cell nuclear antigen (hPCNA) using ÄKTAxpress™
por: Ludwig, Cornelia, et al.
Publicado: (2010)

Untagged $B \to X_{(s+d)}$ gamma CP asymmetry as a probe for new physics
por: Hurth, Tobias, et al.
Publicado: (2003)

Measuring the CKM angle $\gamma$ at LHCb using untagged $B_s \to D \phi$ decays
por: Ricciardi, S
Publicado: (2010)

Use of nitrogen-fixing bacteria to improve agricultural productivity
por: Souza, Emanuel Maltempi, et al.
Publicado: (2014)

KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells
por: Domizi, Pablo, et al.
Publicado: (2019)

A Genome-Wide Assessment of the Role of Untagged Copy Number Variants in Type 1 Diabetes
por: Zanda, Manuela, et al.
Publicado: (2014)

Substrate-Free Untagged Detection of miR393a Using an Ultrasensitive Electrochemical Biosensor
por: Nehra, Anuj, et al.
Publicado: (2022)

Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting
por: Boselli, Daniela, et al.
Publicado: (2023)

Polynomial representations of GLN
por: Green, James A
Publicado: (1980)

Polynomial representations of GLn
por: Green, James A, et al.
Publicado: (2007)

Jet production in untagged $\gamma\gamma$ collisions and single tagged inclusive hadron production at LEP with DELPHI
por: Kapusta, F
Publicado: (1992)

High-throughput Isolation and Characterization of Untagged Membrane Protein Complexes: Outer Membrane Complexes of Desulfovibrio vulgaris
por: Walian, Peter J., et al.
Publicado: (2012)

Structural and functional comparison of hexahistidine tagged and untagged forms of small multidrug resistance protein, EmrE
por: Qazi, S. Junaid S., et al.
Publicado: (2015)

Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy
por: Reif, Andreas, et al.
Publicado: (2018)

Correction: Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting
por: Boselli, Daniela, et al.
Publicado: (2023)

Cytoskeletal Proteins of Actinobacteria
por: Letek, Michal, et al.
Publicado: (2012)

Trans-kingdom rescue of Gln-tRNA(Gln) synthesis in yeast cytoplasm and mitochondria
por: Liao, Chih-Chi, et al.
Publicado: (2012)

The GlnR Regulon in Streptococcus mutans Is Differentially Regulated by GlnR and PmrA
por: Chen, Yi-Ywan M., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_uleg6qpn002rgf6u64hqc4e2nr