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TAMRA/TAMRA Fluorescence Quenching Systems for the Activity Assay of Alkaline Phosphatase

We introduce two types of fluorescence-quenching assay for alkaline phosphatases (APs) by using a carboxytetramethyl-rhodamine (TAMRA)-labeled phosphate-binding tag molecule (TAMRA-Phos-tag). In the first assay, TAMRA-labeled O-phosphorylethanolamine (TAMRA-PEA) was used as an artificial AP-substrat...

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Detalles Bibliográficos
Autores principales:	Shiba, Akio, Kinoshita-Kikuta, Emiko, Kinoshita, Eiji, Koike, Tohru
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5579763/ https://www.ncbi.nlm.nih.gov/pubmed/28809819 http://dx.doi.org/10.3390/s17081877

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