Cargando…
Protein microarray-mediated detection of antienterovirus antibodies in serum
OBJECTIVE: To utilize prokaryotic gene expression and protein microarray to develop and evaluate a sensitive, accurate protein microarray assay for detecting antienterovirus antibodies in serum samples from patients with hand, foot and mouth disease (HFMD). Enterovirus 71 (EV71) and coxsackievirus A...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580062/ https://www.ncbi.nlm.nih.gov/pubmed/26831405 http://dx.doi.org/10.1177/0300060515604981 |
_version_ | 1783260838063243264 |
---|---|
author | Zhang, Aiying Xiu, Bingshui Zhang, Heqiu Li, Ning |
author_facet | Zhang, Aiying Xiu, Bingshui Zhang, Heqiu Li, Ning |
author_sort | Zhang, Aiying |
collection | PubMed |
description | OBJECTIVE: To utilize prokaryotic gene expression and protein microarray to develop and evaluate a sensitive, accurate protein microarray assay for detecting antienterovirus antibodies in serum samples from patients with hand, foot and mouth disease (HFMD). Enterovirus 71 (EV71) and coxsackievirus A16 (CA16), two common causative agents for HFMD, were used for assay development. METHODS: Serum was collected from patients with HFMD and healthy controls. EV71 and CA16 VP1 and VP3 genes were expressed in transfected Escherichia coli; the resultant VP1 and 3 proteins were used in a microarray assay for human serum EV71 and CA16 immunoglobulin (Ig) M and IgG. To validate the microarray assay, serum samples were tested for EV71 IgM using enzyme-linked immunosorbent assay (ELISA). RESULTS: Out of 50 patients with HFMD, EV71 IgM and CA16 IgM was detected in 80% and 44% of serum samples, respectively, using protein microarray, and EV71 IgM was detected in 78% of samples using ELISA. Protein microarray and ELISA showed 100% specificity for EV71-IgM detection. CONCLUSION: The protein microarray assay developed in the present study shows potential as a sensitive technique for detecting EV71 IgM in serum samples from patients with HFMD. |
format | Online Article Text |
id | pubmed-5580062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-55800622017-10-03 Protein microarray-mediated detection of antienterovirus antibodies in serum Zhang, Aiying Xiu, Bingshui Zhang, Heqiu Li, Ning J Int Med Res Research Reports OBJECTIVE: To utilize prokaryotic gene expression and protein microarray to develop and evaluate a sensitive, accurate protein microarray assay for detecting antienterovirus antibodies in serum samples from patients with hand, foot and mouth disease (HFMD). Enterovirus 71 (EV71) and coxsackievirus A16 (CA16), two common causative agents for HFMD, were used for assay development. METHODS: Serum was collected from patients with HFMD and healthy controls. EV71 and CA16 VP1 and VP3 genes were expressed in transfected Escherichia coli; the resultant VP1 and 3 proteins were used in a microarray assay for human serum EV71 and CA16 immunoglobulin (Ig) M and IgG. To validate the microarray assay, serum samples were tested for EV71 IgM using enzyme-linked immunosorbent assay (ELISA). RESULTS: Out of 50 patients with HFMD, EV71 IgM and CA16 IgM was detected in 80% and 44% of serum samples, respectively, using protein microarray, and EV71 IgM was detected in 78% of samples using ELISA. Protein microarray and ELISA showed 100% specificity for EV71-IgM detection. CONCLUSION: The protein microarray assay developed in the present study shows potential as a sensitive technique for detecting EV71 IgM in serum samples from patients with HFMD. SAGE Publications 2016-02-01 2016-04 /pmc/articles/PMC5580062/ /pubmed/26831405 http://dx.doi.org/10.1177/0300060515604981 Text en © The Author(s) 2016 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Research Reports Zhang, Aiying Xiu, Bingshui Zhang, Heqiu Li, Ning Protein microarray-mediated detection of antienterovirus antibodies in serum |
title | Protein microarray-mediated detection of antienterovirus antibodies in serum |
title_full | Protein microarray-mediated detection of antienterovirus antibodies in serum |
title_fullStr | Protein microarray-mediated detection of antienterovirus antibodies in serum |
title_full_unstemmed | Protein microarray-mediated detection of antienterovirus antibodies in serum |
title_short | Protein microarray-mediated detection of antienterovirus antibodies in serum |
title_sort | protein microarray-mediated detection of antienterovirus antibodies in serum |
topic | Research Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580062/ https://www.ncbi.nlm.nih.gov/pubmed/26831405 http://dx.doi.org/10.1177/0300060515604981 |
work_keys_str_mv | AT zhangaiying proteinmicroarraymediateddetectionofantienterovirusantibodiesinserum AT xiubingshui proteinmicroarraymediateddetectionofantienterovirusantibodiesinserum AT zhangheqiu proteinmicroarraymediateddetectionofantienterovirusantibodiesinserum AT lining proteinmicroarraymediateddetectionofantienterovirusantibodiesinserum |