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Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae–Mimosoideae)
BACKGROUND: There is very limited genetic knowledge in Prosopis africana, an important sub-Saharan multi-purpose tree species. Availability of highly polymorphic genetic markers would be helpful for future genetic work. FINDINGS: Leaf samples from 15 trees were used to develop simple sequence repeat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580295/ https://www.ncbi.nlm.nih.gov/pubmed/28859692 http://dx.doi.org/10.1186/s13104-017-2755-x |
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author | Zerbo, Guibien Cleophas Konrad, Heino Ouedraogo, Moussa Geburek, Thomas |
author_facet | Zerbo, Guibien Cleophas Konrad, Heino Ouedraogo, Moussa Geburek, Thomas |
author_sort | Zerbo, Guibien Cleophas |
collection | PubMed |
description | BACKGROUND: There is very limited genetic knowledge in Prosopis africana, an important sub-Saharan multi-purpose tree species. Availability of highly polymorphic genetic markers would be helpful for future genetic work. FINDINGS: Leaf samples from 15 trees were used to develop simple sequence repeat (SSR) markers. Size-selected fragments from genomic DNA were enriched for repeats and the library was analyzed on an Illumina MiSeq platform. Fourteen SSRs were selected and applied in two Burkinabe populations (40 adult trees each). The number of alleles varied from 4 to 20, evenness (effective number of alleles/observed number of alleles) averaged to 0.54 and unbiased heterozygosity ranged from 0.305 to 0.925 over all loci and populations. Null alleles were not detected. CONCLUSIONS: Due to the high level of polymorphism and lack of null alleles the developed SSRs can be effectively employed in population genetic studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13104-017-2755-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5580295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55802952017-09-07 Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae–Mimosoideae) Zerbo, Guibien Cleophas Konrad, Heino Ouedraogo, Moussa Geburek, Thomas BMC Res Notes Short Report BACKGROUND: There is very limited genetic knowledge in Prosopis africana, an important sub-Saharan multi-purpose tree species. Availability of highly polymorphic genetic markers would be helpful for future genetic work. FINDINGS: Leaf samples from 15 trees were used to develop simple sequence repeat (SSR) markers. Size-selected fragments from genomic DNA were enriched for repeats and the library was analyzed on an Illumina MiSeq platform. Fourteen SSRs were selected and applied in two Burkinabe populations (40 adult trees each). The number of alleles varied from 4 to 20, evenness (effective number of alleles/observed number of alleles) averaged to 0.54 and unbiased heterozygosity ranged from 0.305 to 0.925 over all loci and populations. Null alleles were not detected. CONCLUSIONS: Due to the high level of polymorphism and lack of null alleles the developed SSRs can be effectively employed in population genetic studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13104-017-2755-x) contains supplementary material, which is available to authorized users. BioMed Central 2017-08-31 /pmc/articles/PMC5580295/ /pubmed/28859692 http://dx.doi.org/10.1186/s13104-017-2755-x Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Zerbo, Guibien Cleophas Konrad, Heino Ouedraogo, Moussa Geburek, Thomas Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae–Mimosoideae) |
title | Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae–Mimosoideae) |
title_full | Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae–Mimosoideae) |
title_fullStr | Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae–Mimosoideae) |
title_full_unstemmed | Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae–Mimosoideae) |
title_short | Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae–Mimosoideae) |
title_sort | fourteen simple-sequence repeats newly developed for population genetic studies in prosopis africana (fabaceae–mimosoideae) |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580295/ https://www.ncbi.nlm.nih.gov/pubmed/28859692 http://dx.doi.org/10.1186/s13104-017-2755-x |
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