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A simple method to measure CLOCK-BMAL1 DNA binding activity in tissue and cell extracts

The proteins CLOCK and BMAL1 form a heterodimeric transcription factor essential to circadian rhythms in mammals. Daily rhythms of CLOCK-BMAL1 DNA binding activity are known to oscillate with target gene expression in vivo. Here we present a highly sensitive assay that recapitulates native CLOCK-BM...

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Detalles Bibliográficos
Autores principales:	Gillessen, Maud, Kwak, Pieter Bas, Tamayo, Alfred
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	F1000Research 2017
Materias:	Method Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580408/ https://www.ncbi.nlm.nih.gov/pubmed/28928952 http://dx.doi.org/10.12688/f1000research.11685.2

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