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Novelty and fear conditioning induced gene expression in high and low states of anxiety

Emotional states influence how stimuli are interpreted. High anxiety states in humans lead to more negative, threatening interpretations of novel information, typically accompanied by activation of the amygdala. We developed a handling protocol that induces long-lasting high and low anxiety-like sta...

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Detalles Bibliográficos
Autores principales: Donley, Melanie P., Rosen, Jeffrey B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580523/
https://www.ncbi.nlm.nih.gov/pubmed/28814471
http://dx.doi.org/10.1101/lm.044289.116
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author Donley, Melanie P.
Rosen, Jeffrey B.
author_facet Donley, Melanie P.
Rosen, Jeffrey B.
author_sort Donley, Melanie P.
collection PubMed
description Emotional states influence how stimuli are interpreted. High anxiety states in humans lead to more negative, threatening interpretations of novel information, typically accompanied by activation of the amygdala. We developed a handling protocol that induces long-lasting high and low anxiety-like states in rats to explore the role of state anxiety on brain activation during exposure to a novel environment and fear conditioning. In situ hybridization of the inducible transcription factor Egr-1 found increased gene expression in the lateral nucleus of the amygdala (LA) following exposure to a novel environment and contextual fear conditioning in high anxiety-like rats. In contrast, low state anxiety-like rats did not generate Egr-1 increases in LA when placed in a novel chamber. Egr-1 expression was also examined in the dorsal hippocampus and prefrontal cortex. In CA1 of the hippocampus and medial prefrontal cortex (mPFC), Egr-1 expression increased in response to novel context exposure and fear conditioning, independent of state anxiety level. Furthermore, in mPFC, Egr-1 in low anxiety-like rats was increased more with fear conditioning than novel exposure. The current series of experiments show that brain areas involved in fear and anxiety-like states do not respond uniformly to novelty during high and low states of anxiety.
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spelling pubmed-55805232018-09-01 Novelty and fear conditioning induced gene expression in high and low states of anxiety Donley, Melanie P. Rosen, Jeffrey B. Learn Mem Research Emotional states influence how stimuli are interpreted. High anxiety states in humans lead to more negative, threatening interpretations of novel information, typically accompanied by activation of the amygdala. We developed a handling protocol that induces long-lasting high and low anxiety-like states in rats to explore the role of state anxiety on brain activation during exposure to a novel environment and fear conditioning. In situ hybridization of the inducible transcription factor Egr-1 found increased gene expression in the lateral nucleus of the amygdala (LA) following exposure to a novel environment and contextual fear conditioning in high anxiety-like rats. In contrast, low state anxiety-like rats did not generate Egr-1 increases in LA when placed in a novel chamber. Egr-1 expression was also examined in the dorsal hippocampus and prefrontal cortex. In CA1 of the hippocampus and medial prefrontal cortex (mPFC), Egr-1 expression increased in response to novel context exposure and fear conditioning, independent of state anxiety level. Furthermore, in mPFC, Egr-1 in low anxiety-like rats was increased more with fear conditioning than novel exposure. The current series of experiments show that brain areas involved in fear and anxiety-like states do not respond uniformly to novelty during high and low states of anxiety. Cold Spring Harbor Laboratory Press 2017-09 /pmc/articles/PMC5580523/ /pubmed/28814471 http://dx.doi.org/10.1101/lm.044289.116 Text en © 2017 Donley and Rosen; Published by Cold Spring Harbor Laboratory Press https://creativecommons.org/licenses/by-nc/4.0/This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first 12 months after the full-issue publication date (see http://learnmem.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Research
Donley, Melanie P.
Rosen, Jeffrey B.
Novelty and fear conditioning induced gene expression in high and low states of anxiety
title Novelty and fear conditioning induced gene expression in high and low states of anxiety
title_full Novelty and fear conditioning induced gene expression in high and low states of anxiety
title_fullStr Novelty and fear conditioning induced gene expression in high and low states of anxiety
title_full_unstemmed Novelty and fear conditioning induced gene expression in high and low states of anxiety
title_short Novelty and fear conditioning induced gene expression in high and low states of anxiety
title_sort novelty and fear conditioning induced gene expression in high and low states of anxiety
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580523/
https://www.ncbi.nlm.nih.gov/pubmed/28814471
http://dx.doi.org/10.1101/lm.044289.116
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