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Maternal mRNAs with distinct 3′ UTRs define the temporal pattern of Ccnb1 synthesis during mouse oocyte meiotic maturation

The final stages of female gamete maturation occur in the virtual absence of transcription, with gene expression driven by a program of selective unmasking, translation, and degradation of maternal mRNAs. Here we demonstrate that the timing of Ccnb1 mRNA translation in mouse oocytes is dependent on...

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Autores principales: Yang, Ye, Yang, Cai-Rong, Han, Seung Jin, Daldello, Enrico Maria, Cho, Ara, Martins, Joao P. Sousa, Xia, Guoliang, Conti, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580652/
https://www.ncbi.nlm.nih.gov/pubmed/28808066
http://dx.doi.org/10.1101/gad.296871.117
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author Yang, Ye
Yang, Cai-Rong
Han, Seung Jin
Daldello, Enrico Maria
Cho, Ara
Martins, Joao P. Sousa
Xia, Guoliang
Conti, Marco
author_facet Yang, Ye
Yang, Cai-Rong
Han, Seung Jin
Daldello, Enrico Maria
Cho, Ara
Martins, Joao P. Sousa
Xia, Guoliang
Conti, Marco
author_sort Yang, Ye
collection PubMed
description The final stages of female gamete maturation occur in the virtual absence of transcription, with gene expression driven by a program of selective unmasking, translation, and degradation of maternal mRNAs. Here we demonstrate that the timing of Ccnb1 mRNA translation in mouse oocytes is dependent on the presence of transcripts with different 3′ untranslated regions (UTRs). This 3′ UTR heterogeneity directs distinct temporal patterns of translational activation or repression. Inclusion or exclusion of cis-acting elements is responsible for these divergent regulations. Our findings reveal an additional layer of translation control through alternative polyadenylation usage required to fine-tune the timing of meiosis progression.
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spelling pubmed-55806522018-01-01 Maternal mRNAs with distinct 3′ UTRs define the temporal pattern of Ccnb1 synthesis during mouse oocyte meiotic maturation Yang, Ye Yang, Cai-Rong Han, Seung Jin Daldello, Enrico Maria Cho, Ara Martins, Joao P. Sousa Xia, Guoliang Conti, Marco Genes Dev Research Communication The final stages of female gamete maturation occur in the virtual absence of transcription, with gene expression driven by a program of selective unmasking, translation, and degradation of maternal mRNAs. Here we demonstrate that the timing of Ccnb1 mRNA translation in mouse oocytes is dependent on the presence of transcripts with different 3′ untranslated regions (UTRs). This 3′ UTR heterogeneity directs distinct temporal patterns of translational activation or repression. Inclusion or exclusion of cis-acting elements is responsible for these divergent regulations. Our findings reveal an additional layer of translation control through alternative polyadenylation usage required to fine-tune the timing of meiosis progression. Cold Spring Harbor Laboratory Press 2017-07-01 /pmc/articles/PMC5580652/ /pubmed/28808066 http://dx.doi.org/10.1101/gad.296871.117 Text en © 2017 Yang et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Yang, Ye
Yang, Cai-Rong
Han, Seung Jin
Daldello, Enrico Maria
Cho, Ara
Martins, Joao P. Sousa
Xia, Guoliang
Conti, Marco
Maternal mRNAs with distinct 3′ UTRs define the temporal pattern of Ccnb1 synthesis during mouse oocyte meiotic maturation
title Maternal mRNAs with distinct 3′ UTRs define the temporal pattern of Ccnb1 synthesis during mouse oocyte meiotic maturation
title_full Maternal mRNAs with distinct 3′ UTRs define the temporal pattern of Ccnb1 synthesis during mouse oocyte meiotic maturation
title_fullStr Maternal mRNAs with distinct 3′ UTRs define the temporal pattern of Ccnb1 synthesis during mouse oocyte meiotic maturation
title_full_unstemmed Maternal mRNAs with distinct 3′ UTRs define the temporal pattern of Ccnb1 synthesis during mouse oocyte meiotic maturation
title_short Maternal mRNAs with distinct 3′ UTRs define the temporal pattern of Ccnb1 synthesis during mouse oocyte meiotic maturation
title_sort maternal mrnas with distinct 3′ utrs define the temporal pattern of ccnb1 synthesis during mouse oocyte meiotic maturation
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580652/
https://www.ncbi.nlm.nih.gov/pubmed/28808066
http://dx.doi.org/10.1101/gad.296871.117
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