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Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA
X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom C...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580792/ https://www.ncbi.nlm.nih.gov/pubmed/28862633 http://dx.doi.org/10.1107/S160057751701030X |
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author | Kubo, Minoru Nango, Eriko Tono, Kensuke Kimura, Tetsunari Owada, Shigeki Song, Changyong Mafuné, Fumitaka Miyajima, Ken Takeda, Yoshihiro Kohno, Jun-ya Miyauchi, Naoya Nakane, Takanori Tanaka, Tomoyuki Nomura, Takashi Davidsson, Jan Tanaka, Rie Murata, Michio Kameshima, Takashi Hatsui, Takaki Joti, Yasumasa Neutze, Richard Yabashi, Makina Iwata, So |
author_facet | Kubo, Minoru Nango, Eriko Tono, Kensuke Kimura, Tetsunari Owada, Shigeki Song, Changyong Mafuné, Fumitaka Miyajima, Ken Takeda, Yoshihiro Kohno, Jun-ya Miyauchi, Naoya Nakane, Takanori Tanaka, Tomoyuki Nomura, Takashi Davidsson, Jan Tanaka, Rie Murata, Michio Kameshima, Takashi Hatsui, Takaki Joti, Yasumasa Neutze, Richard Yabashi, Makina Iwata, So |
author_sort | Kubo, Minoru |
collection | PubMed |
description | X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented. |
format | Online Article Text |
id | pubmed-5580792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-55807922017-09-05 Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA Kubo, Minoru Nango, Eriko Tono, Kensuke Kimura, Tetsunari Owada, Shigeki Song, Changyong Mafuné, Fumitaka Miyajima, Ken Takeda, Yoshihiro Kohno, Jun-ya Miyauchi, Naoya Nakane, Takanori Tanaka, Tomoyuki Nomura, Takashi Davidsson, Jan Tanaka, Rie Murata, Michio Kameshima, Takashi Hatsui, Takaki Joti, Yasumasa Neutze, Richard Yabashi, Makina Iwata, So J Synchrotron Radiat Short Communications X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented. International Union of Crystallography 2017-08-22 /pmc/articles/PMC5580792/ /pubmed/28862633 http://dx.doi.org/10.1107/S160057751701030X Text en © Minoru Kubo et al. 2017 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/ |
spellingShingle | Short Communications Kubo, Minoru Nango, Eriko Tono, Kensuke Kimura, Tetsunari Owada, Shigeki Song, Changyong Mafuné, Fumitaka Miyajima, Ken Takeda, Yoshihiro Kohno, Jun-ya Miyauchi, Naoya Nakane, Takanori Tanaka, Tomoyuki Nomura, Takashi Davidsson, Jan Tanaka, Rie Murata, Michio Kameshima, Takashi Hatsui, Takaki Joti, Yasumasa Neutze, Richard Yabashi, Makina Iwata, So Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA |
title | Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA |
title_full | Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA |
title_fullStr | Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA |
title_full_unstemmed | Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA |
title_short | Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA |
title_sort | nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at sacla |
topic | Short Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580792/ https://www.ncbi.nlm.nih.gov/pubmed/28862633 http://dx.doi.org/10.1107/S160057751701030X |
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