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Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA

X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom C...

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Autores principales: Kubo, Minoru, Nango, Eriko, Tono, Kensuke, Kimura, Tetsunari, Owada, Shigeki, Song, Changyong, Mafuné, Fumitaka, Miyajima, Ken, Takeda, Yoshihiro, Kohno, Jun-ya, Miyauchi, Naoya, Nakane, Takanori, Tanaka, Tomoyuki, Nomura, Takashi, Davidsson, Jan, Tanaka, Rie, Murata, Michio, Kameshima, Takashi, Hatsui, Takaki, Joti, Yasumasa, Neutze, Richard, Yabashi, Makina, Iwata, So
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580792/
https://www.ncbi.nlm.nih.gov/pubmed/28862633
http://dx.doi.org/10.1107/S160057751701030X
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author Kubo, Minoru
Nango, Eriko
Tono, Kensuke
Kimura, Tetsunari
Owada, Shigeki
Song, Changyong
Mafuné, Fumitaka
Miyajima, Ken
Takeda, Yoshihiro
Kohno, Jun-ya
Miyauchi, Naoya
Nakane, Takanori
Tanaka, Tomoyuki
Nomura, Takashi
Davidsson, Jan
Tanaka, Rie
Murata, Michio
Kameshima, Takashi
Hatsui, Takaki
Joti, Yasumasa
Neutze, Richard
Yabashi, Makina
Iwata, So
author_facet Kubo, Minoru
Nango, Eriko
Tono, Kensuke
Kimura, Tetsunari
Owada, Shigeki
Song, Changyong
Mafuné, Fumitaka
Miyajima, Ken
Takeda, Yoshihiro
Kohno, Jun-ya
Miyauchi, Naoya
Nakane, Takanori
Tanaka, Tomoyuki
Nomura, Takashi
Davidsson, Jan
Tanaka, Rie
Murata, Michio
Kameshima, Takashi
Hatsui, Takaki
Joti, Yasumasa
Neutze, Richard
Yabashi, Makina
Iwata, So
author_sort Kubo, Minoru
collection PubMed
description X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.
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spelling pubmed-55807922017-09-05 Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA Kubo, Minoru Nango, Eriko Tono, Kensuke Kimura, Tetsunari Owada, Shigeki Song, Changyong Mafuné, Fumitaka Miyajima, Ken Takeda, Yoshihiro Kohno, Jun-ya Miyauchi, Naoya Nakane, Takanori Tanaka, Tomoyuki Nomura, Takashi Davidsson, Jan Tanaka, Rie Murata, Michio Kameshima, Takashi Hatsui, Takaki Joti, Yasumasa Neutze, Richard Yabashi, Makina Iwata, So J Synchrotron Radiat Short Communications X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented. International Union of Crystallography 2017-08-22 /pmc/articles/PMC5580792/ /pubmed/28862633 http://dx.doi.org/10.1107/S160057751701030X Text en © Minoru Kubo et al. 2017 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Short Communications
Kubo, Minoru
Nango, Eriko
Tono, Kensuke
Kimura, Tetsunari
Owada, Shigeki
Song, Changyong
Mafuné, Fumitaka
Miyajima, Ken
Takeda, Yoshihiro
Kohno, Jun-ya
Miyauchi, Naoya
Nakane, Takanori
Tanaka, Tomoyuki
Nomura, Takashi
Davidsson, Jan
Tanaka, Rie
Murata, Michio
Kameshima, Takashi
Hatsui, Takaki
Joti, Yasumasa
Neutze, Richard
Yabashi, Makina
Iwata, So
Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA
title Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA
title_full Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA
title_fullStr Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA
title_full_unstemmed Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA
title_short Nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at SACLA
title_sort nanosecond pump–probe device for time-resolved serial femtosecond crystallography developed at sacla
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580792/
https://www.ncbi.nlm.nih.gov/pubmed/28862633
http://dx.doi.org/10.1107/S160057751701030X
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